膀胱出口部分梗阻后的逼尿肌过度活动与雌性 Wistar 大鼠尿中三磷酸腺苷水平过高有关。

IF 1.8 3区医学 Q3 UROLOGY & NEPHROLOGY

International Neurourology Journal Pub Date : 2024-02-01 Epub Date: 2024-02-29 DOI:10.5213/inj.2346196.098

Luís Vale, Francisco Cruz, Ana Charrua

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引用次数: 0

摘要

目的：膀胱出口梗阻（BOO）通常会导致逼尿肌过度活动（DO）。在本研究中，我们对之前获得的数据进行了事后分析，研究膀胱出口梗阻初期发生的DO是否与尿液中三磷酸腺苷（ATP）水平的变化有关：方法：成年雌性 Wistar 大鼠接受部分 BOO（pBOO）或假阻塞。在 pBOO 后 3 天或 15 天进行膀胱测定，并收集排出的生理盐水以测定 ATP。用 Shapiro-Wilk 检验法检测了正态性。假手术动物术后 15 天的排尿收缩（VCs）平均频率，加上或减去 3 个标准差，代表正常范围。统计分析采用卡方检验和曼-惠特尼检验：VC频率大于或等于0.9 VCs/min表示有DO。63%的动物在pBOO后3天观察到DO，33%的动物在pBOO后15天观察到DO。与无 DO 的大鼠相比，有 DO 的大鼠 ATP 水平明显更高：结论：BOO初期出现的DO表型与相对较高的尿ATP水平有关。

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Detrusor Overactivity After Partial Bladder Outlet Obstruction Is Associated With High Urinary Adenosine Triphosphate Levels in Female Wistar Rats.

Purpose: Bladder outlet obstruction (BOO) commonly causes detrusor overactivity (DO). In this study, a post hoc analysis of previous obtained data, we investigate if DO occurring in initial phases of BOO is associated with changes in urinary adenosine triphosphate (ATP) levels.

Methods: Adult female Wistar rats were submitted to partial BOO (pBOO) or to sham obstruction. Cystometry was performed at 3 or 15 days after pBOO and saline voided was collected for ATP determination. Normality was tested using Shapiro-Wilk test. The mean frequency of voiding contractions (VCs) of the sham-operated animals at 15 days after surgery, plus or minus 3 standard deviations, was used to represent the normal range. Statistical analyses were performed using the chi-square and Mann-Whitney tests.

Results: DO was indicated by a VC frequency greater than or equal to 0.9 VCs/min. DO was observed in 63% of animals at 3 days and in 33% at 15 days following pBOO. ATP levels were significantly higher in rats with DO compared to those without DO.

Conclusion: The DO phenotype, occurring in the initial phases of BOO, is associated with comparatively high urinary ATP levels.

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来源期刊

International Neurourology Journal UROLOGY & NEPHROLOGY-

CiteScore

4.40

自引率

21.70%

发文量

审稿时长

4 weeks

期刊介绍： The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that provide the most significant and promising achievements in the fields of clinical neurourology and fundamental science. Specifically, fundamental science includes the most influential research papers from all fields of science and technology, revolutionizing what physicians and researchers practicing the art of neurourology worldwide know. Thus, we welcome valuable basic research articles to introduce cutting-edge translational research of fundamental sciences to clinical neurourology. In the editorials, urologists will present their perspectives on these articles. The original mission statement of the INJ was published on October 12, 1997. INJ provides authors a fast review of their work and makes a decision in an average of three to four weeks of receiving submissions. If accepted, articles are posted online in fully citable form. Supplementary issues will be published interim to quarterlies, as necessary, to fully allow berth to accept and publish relevant articles.