蚕丝纤维素/聚（乳酸-共羟基乙酸）纳米纤维涂层对骨质疏松症用多孔磷酸钙基复合材料的影响

IF 2.9 4区医学 Q1 Medicine

Journal of biomedical nanotechnology Pub Date : 2024-03-01 DOI:10.1166/jbn.2024.3785

Qiao Ren, Qinggui Li, Ligang Qian

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引用次数: 0

摘要

本研究观察了丝纤维素/聚乳酸-乙醇酸（SF/PLGA）纳米纤维膜三维包覆多孔磷酸钙基复合材料能否促进成骨细胞增殖，从而改善骨质疏松症。利用SF/PLGA纳米纤维膜三维包覆多孔磷酸钙基复合材料，分析了成骨细胞的增殖、Western blot检测hFOB1.19的表达、CCK-8检测hFOB1.19的活性变化。用电子显微镜观察其形态。茜素红染色表明，SF/PLGA 纳米纤维膜三维包覆多孔磷酸钙基复合材料可促进成骨分化，并上调成骨相关基因的表达。它抑制了成脂分化和成脂基因的表达。SF/PLGA 复合材料能促进成骨细胞 hFOB1.19 的活力，上调 GLUT3，下调 hFOB1.19 中各种凋亡相关蛋白的表达。SF/PLGA复合材料能降低成骨细胞的凋亡效率，促进成骨细胞增殖，抑制细胞凋亡，从而改善骨质疏松症。

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The Effect of Silk Fibroin/Poly(Lactic-Co-Glycolic Acid) Nanofiber Coating on Porous Calcium Phosphate-Based Composites for Osteoporosis Application

This study observed whether the silk fibroin/poly lactic-co-glycolic acid (SF/PLGA) nanofiber membrane three-dimensionally coated with porous calcium phosphate-based composite can promote the proliferation of osteoblasts, thereby improving osteoporosis. The SF/PLGA nanofiber membrane was used to three-dimensionally coated with the porous calcium phosphate-based composite to analyze proliferation of osteoblasts, hFOB1.19 expressions by Western blot, activity change of hFOB1.19 by CCK-8 assay. The morphology was observed with an electron microscope. Alizarin Red staining showed that the SF/PLGA nanofiber membrane three-dimensionally coated with porous calcium phosphatebased composite promoted osteogenic differentiation and upregulated the expression of osteogenic related genes. It inhibited adipogenic differentiation and the expression of adipogenic genes. SF/PLGA composite material promoted the viability of osteoblasts hFOB1.19, upregulatedGLUT3, downregulated the expression of various apoptosis-related proteins in hFOB1.19. SF/PLGA composite material can reduce the apoptosis efficiency of osteoblasts, promote the proliferation of osteoblasts, and inhibit apoptosis, thereby improving osteoporosis.

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来源期刊

Journal of biomedical nanotechnology 医学-纳米科技

CiteScore

4.30

自引率

17.20%

发文量

145

审稿时长

2.3 months

期刊介绍： Information not localized