姜黄和胡椒对耐甲氧西林金黄色葡萄球菌和感染性血管生成的影响

IF 0.5 4区医学

Journal of Biobased Materials and Bioenergy Pub Date : 2024-03-01 DOI:10.1166/jbmb.2024.2363

Ghulam Jilany Khan, Zil E. Humma, M. O. Omer, Adeel Sattar, I. Altaf, Zhu Chen, Song Li, Hiui Chen, Yan Deng, Nongyue He

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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌是细菌传播的传染病中一种具有挑战性的微生物，由于病原体具有抗药性，因此极难治疗。因此，治疗与 MRSA 相关的感染总是需要新的药物。从植物中提取的精华早在人类早期就被用于医药实践。目前的研究旨在发现黑胡椒和莪术作为单独或联合药剂对细菌菌株和相关感染性血管生成的治疗作用。用琼脂井扩散法计算出的姜黄和胡椒的单药 MIC 分别为 1.56 毫克/毫升和 12.5 毫克/毫升，而姜黄和胡椒联合用药的 MIC 为 0.062 毫克/毫升，表明联合用药的有效 MIC 显著降低。FICI 值为 0.034 ≤ 0.5，证实了组合提取物的协同抗菌活性。MTT 试验表明，姜黄、胡椒和它们的组合提取物的细胞存活率和 IC50 值分别为 69%、41%、80% 和 38.3 ± 2.82、9.92 ± 1.84、62 ± 5.32，表明治疗剂量无毒。萃取物的进一步抗菌活性显示了对 MRSA 的抗菌效果。通过 CAM 试验观察了对感染性血管生成的影响，结果显示当姜黄和胡椒联合使用时，对新生血管生成的抑制作用比单独使用时更强，这进一步证明了胡椒的协同作用。和瓜蒌提取物通过干扰感染性血管生成机制和杀菌作用，以协同方式对抗 MRSA 感染的新的潜在效用；建议进一步研究分离出具有杀菌和抗感染抗血管生成活性的活性化合物，以作为现代医学领域的治疗药物。

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Effects of Curcuma longa L. and Piper nigrum L. Against Methicillin Resistant Staphylococcus aureus and Infectious Angiogenesis

Methicillin drug resistant Staphylococcus aureus is a challenging microbe among bacteria borne infectious diseases and is extremely difficult to treat because of resistive nature of pathogen. Therefore, new agents are always needed to treat MRSA associated infections. Extracts from plants have been in medicinal practice since early ages of mankind. Current research is meant to discern the therapeutic aspects of Piper nigrum L. and Curcuma longa L. as solo or in combinatory medicinal agent against bacterial strains and associated infectious angiogenesis. The individual MICs of Curcuma longa and Piper nigrum calculated by agar well diffusion method were 1.56 mg/mL and 12.5 mg/mL respectively whereas the MIC of the combination of Curcuma longa and Piper nigrum was 0.062 mg/mL, indicating significant decrease in effective MIC in combination. FICI value was 0.034 ≤ 0.5 that confirmed the synergistic antibacterial activity of the combination extract. MTT assay showed that the cell survival percentage and IC50 values of Curcuma longa L., Piper nigrum L. and their combination were 69%, 41%, 80% and 38.3 ± 2.82, 9.92 ± 1.84, 62 ± 5.32 respectively indicating therapeutic doses were non-toxic. Further antibacterial activity of extracts showed promising antibacterial effects against MRSA. Effects on infectious angiogenesis were observed by CAM assay which revealed that when Curcuma longa L. and Piper nigrum L. were used in combination, inhibition of neovascularization was more promising as compared to individual effect which further testified the synergistic effect of Piper nigrum L. Present study accumulatively reports the novel potential utility of Curcuma longa L. and Piper nigrum L. extracts in a synergistic way against MRSA infection by interfering the mechanism of infectious angiogenesis and bactericidal action; suggesting further investigation to isolate active compounds for bactericidal and anti-infectious antiangiogenic activities that may serve as therapeutic agents in the modern field of medicine.

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来源期刊

Journal of Biobased Materials and Bioenergy 工程技术-材料科学：生物材料

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审稿时长

6 months