纤维素纳米纸携带的顺式铂和 CXCR4 siRNA 限制了乳腺癌细胞的生物效应

IF 2.9 4区 医学 Q1 Medicine
Yang Zhang, Jianfei Wang, Hao Dai, Ning Li, Fen Hu, Bolin Zhang, Yiming Yang, Haifeng Cai, Jinyin Yan
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引用次数: 0

摘要

纤维素纳米纸(CNP)是一种由纤维素纳米纤维组成的平面可折叠薄膜材料。纤维素纳米纸是以纳米纤维素(NC)为基本单元,具有优异的力学性能、热性能、光学性能等特点,是一种高性能的新型材料。本研究探讨了顺铂和以 CNP 为载体的 CXCR4 siRNA 抑制乳腺癌细胞生物学效应的机制。建立并鉴定了以 CNP 为载体的 CXCR4 siRNA。将 MCF-7 细胞随机分为对照组、si-CXCR4 组、顺铂组和联合组。通过显著敲除 CXCR4,抑制了 CXCR4 的 mRNA 和蛋白表达。细胞增殖受到抑制,细胞凋亡得到促进。E-cadherin 的表达增加,Zeb1 的表达减少。侵袭能力受到抑制。该作用在联合组中更为显著。顺铂和 CNP 所携带的 CXCR4 siRNA 抑制了乳腺癌细胞的增殖并促进了细胞凋亡。EMT和侵袭的发生也因此受到抑制。乳腺癌对药物的敏感性提高了。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cis-Platinum and CXCR4 siRNA Carried with Cellulose Nanopaper Restrain the Biological Effect of Breast Carcinoma Cells
Cellulose nanopaper (CNP) is a kind of flat foldable film material composed of cellulose nanofibers. Cellulose nanopaper is based on nanocellulose (NC) as the basic unit, with excellent mechanical properties, thermal properties, optical properties and other characteristics, is a high-performance new material. This study discusses the mechanism of cis-platinum and CXCR4 siRNA carried with CNP in restraining the biological effect of breast carcinoma cells. CXCR4 siRNA carried with CNP was established and identified. The MCF-7 cells were divided into control set, si-CXCR4 set, cis-platinum set and united set randomly. The proliferative and apoptotic activity, CXCR4 presentation, presentation of EMT, and invasive ability were detected. mRNA and protein expression of CXCR4 was restrained by knockdown of CXCR4 notably. The proliferation was restrained and apoptosis was prompted. Expression of E-cadherin was increased and Zeb1 presentation was reduced. The invasive ability was restrained. The action was more notable in united set. Breast carcinoma cell proliferation was restrained and the apoptosis was prompted by cis-platinum and CXCR4 siRNA carried with CNP. The occurrence of EMT and invasion were therefore restrained. The sensibility of breast carcinoma on drugs was elevated.
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来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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