Zequn Zhang, Chen Meng, Zhigong Wang, Yan Huang, X. Lü
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引用次数: 0
摘要
我们课题组之前提出了基于微电极阵列(MEA)的电压阈值测量方法(VTMM),并利用VTMM证明PC12细胞作为一种较简单的细胞培养物,可以作为一种替代细胞模型来研究外界因素对神经元细胞毒性和电兴奋性的影响。然而,目前还没有将 VTMM 与常用的 MEA 神经信号分析方法进行比较,也没有研究人员使用常用的分析方法来研究 PC12 准神经元网络的自发电信号。本研究旨在比较两种基于 MEA 的神经信号分析方法的特点和结果。该研究首次利用 MEA 分析方法研究了 PC12 网络在暴露于不同浓度的银纳米粒子不同持续时间后尖峰频率的变化。研究发现,由于 PC12 网络的自发活动强度较低,常用的分析方法并不适合研究 PC12 网络的电活动。在银纳米颗粒的作用下,VTMM的结果与常用的MEA分析方法一致,且VTMM具有适用于更多种类的神经元网络、培养时间短、数据分析简洁快速等优点。
The Effect of Silver Nanoparticles on the Electrical Activity of PC12 Quasi-Neuronal Networks and the Comparison of Two Microelectrode Array-Based Neural Signal Analysis Methods
Our research group previously proposed the voltage thresholdmeasurement method (VTMM) based on the microelectrode array (MEA), and used VTMM to prove that PC12 cells, as a simpler cell culture, can be used as an alternative cell model to examine the effects of external factors on cytotoxicity
and electrical excitability of neurons. However, there is no comparison between VTMM and the commonly used MEA neural signal analysis method, and no researchers have used the commonly used analysis method to study the spontaneous electrical signals of PC12 quasi-neuronal networks. This research
aims to compare the characteristics and results of two MEA-based neural signal analysis methods. For the first time, the changes in spike frequency of the PC12 networks after exposure to various concentrations of silver nanoparticles for different durations was studied by means of MEA analysis
method. It is found that due to the low intensity of spontaneous activity of PC12 networks, the commonly used analysis method is not suitable for the study of electrical activity of PC12 networks. Under the action of silver nanoparticles, the results of VTMM are consistent with those of the
commonly used MEA analysis method, and VTMM has the advantages of being suitable for more kinds of neuron networks, short culture time, concise and rapid data analysis.