禽源性益生菌乳酸菌对热应激鸡巨噬细胞功能活性的调节作用

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Khaled Abdelaziz, Thandi Nixon, Annie Joye, Hosni Hassan, Mohammadali Alizadeh, Shayan Sharif, R. Kulkarni
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引用次数: 0

摘要

本研究调查了乳酸菌在减轻热应激对鸡巨噬细胞功能活动的负面影响方面的潜在作用。将类似于巨噬细胞的 MQ-NCSU 细胞置于 40oC 或 44oC 的温度下,在有或没有单一或混合的不同禽源性乳酸杆菌(包括动物乳杆菌、嗜酸乳杆菌、纽崔莱乳杆菌和脆乳杆菌)的情况下进行培养。通过测量一氧化氮(NO)的产生、吞噬活性以及细胞因子、趋化因子和 Toll 样受体(TLRs)的转录水平来评估巨噬细胞的活化情况。暴露于热应激的巨噬细胞表现出一氧化氮产生增加,白细胞介素(IL)-1β和IL-12p40表达减少,TLR2和TLR4表达升高,而巨噬细胞的吞噬活性没有明显变化。相反,用益生菌乳酸杆菌处理巨噬细胞可以抵消与热应激相关的影响。这表现在巨噬细胞的吞噬活性、NO 的产生和 IL-1β、IL-12p40、IL-18 和 CXCL8 的表达明显增强,同时 TLR2 和 TLR4 的表达减少。这些研究结果表明,益生菌乳酸菌可以减轻热应激对先天免疫系统的负面影响。不过,还需要进一步的研究来验证在体内模型中观察到的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulation of Functional Activity of Heat-Stressed Chicken Macrophages by Poultry-Derived Probiotic Lactobacilli
This study investigated the potential role of lactobacilli in mitigating the negative effects of heat stress on the functional activity of chicken macrophages. Macrophage-like MQ-NCSU cells were incubated at 40oC or 44oC in the presence or absence of a single or a mixture of different poultry-derived Lactobacillus species, including L. animalis, L. acidophilus, L. reuteri, and L. crispatus. Macrophage activation was evaluated by measuring nitric oxide (NO) production, phagocytic activity, and the transcription levels of cytokines, chemokine, and Toll-like receptors (TLRs). Macrophages exposed to heat stress exhibited increased production of NO, diminished expression of interleukin (IL)-1β and IL-12p40, and elevated expression of TLR2 and TLR4, whereas no significant alterations in the phagocytic activity of macrophages were observed. Conversely, treatment of macrophages with probiotic lactobacilli counteracted the effects associated with heat stress. This was evidenced by a notable enhancement in macrophage phagocytic activity, NO production and expression of IL-1β, IL-12p40, IL-18, and CXCL8, coupled with a reduction in TLR2 and TLR4 expression. These findings suggest that probiotic lactobacilli could be given to chickens to mitigate the negative effects of heat stress on the innate immune system. However, further studies are required to validate the observed effects in an in vivo model.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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