茄科植物多糖的成分和有丝分裂活性

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050093

E. A. Generalov, L. V. Yakovenko

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引用次数: 0

摘要

摘要从茄科植物Solanum tuberosum L.的水提取物中分离出STP多糖，并通过离子交换色谱和凝胶过滤进行纯化。用凝胶穿透色谱法和高效液相色谱法测定了其分子质量，并用高效液相色谱法和气相色谱法（火焰离子化检测器和毛细管柱）分析了其单糖组成。结果表明，STP 多糖由半乳糖和阿拉伯糖组成，分别占 37.5%和 23.5%，此外还有尿酸（9.7%）、葡萄糖单糖残基（15%）和蛋白质（至少 9%）。STP 的分子质量为 70 kDa。红外-傅里叶光谱法用于分析 STP 的结构。提取多糖的有丝分裂活性与脂多糖的活性相当。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.

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The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.

The STP polysaccharide was isolated from an aqueous extract of Solanum tuberosum L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.