{"title":"拟南芥中的氧化 ALLENE OXIDE SYNTHASE (AOS) 通过新型 JA 相关调控途径诱导花瓣衰老","authors":"Liuqing Wu, Kaiqi Wang, Mengyi Chen, Wenxin Su, Zheng Liu, Xiaoying Guo, Mengqian Ma, Shuangjie Qian, Yuqi Deng, Haihan Wang, Chanjuan Mao, Zaibao Zhang, Xiaofeng Xu","doi":"10.1007/s12298-024-01425-w","DOIUrl":null,"url":null,"abstract":"<p>Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in <i>Arabidopsis</i> was investigated. We showed that petal senescence in <i>aos</i> mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in <i>dad1</i> and <i>coi1</i> mutants. However, <i>MYB21/24</i> and <i>MYC2/3/4</i>, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that <i>AOS</i> altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, <i>bHLH13</i>, <i>bHLH17</i> and <i>URH2</i> were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in <i>Arabidopsis</i>. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis\",\"authors\":\"Liuqing Wu, Kaiqi Wang, Mengyi Chen, Wenxin Su, Zheng Liu, Xiaoying Guo, Mengqian Ma, Shuangjie Qian, Yuqi Deng, Haihan Wang, Chanjuan Mao, Zaibao Zhang, Xiaofeng Xu\",\"doi\":\"10.1007/s12298-024-01425-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in <i>Arabidopsis</i> was investigated. We showed that petal senescence in <i>aos</i> mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in <i>dad1</i> and <i>coi1</i> mutants. However, <i>MYB21/24</i> and <i>MYC2/3/4</i>, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that <i>AOS</i> altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, <i>bHLH13</i>, <i>bHLH17</i> and <i>URH2</i> were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in <i>Arabidopsis</i>. 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引用次数: 0
摘要
花朵对有花植物的繁殖至关重要,花朵的衰老对植物与动物之间的相互作用以及授粉都有重大影响。花瓣衰老是花发育的最后阶段,受激素和基因的调控。其中,茉莉酸(JA)已成为花瓣衰老的主要因素,但其分子机制仍然难以捉摸。本文研究了 JA 在拟南芥花瓣衰老中的作用。我们发现,aos 突变体的花瓣衰老显著延迟,这也影响了花瓣细胞的大小和增殖。在 dad1 和 coi1 突变体中也观察到类似的花瓣衰老明显延迟现象。然而,已知的 JA 在花发育过程中的下游调控因子 MYB21/24 和 MYC2/3/4 在花瓣衰老中没有发挥作用。这表明 JA 是通过调节其他未知转录因子来调控花瓣衰老的。转录组分析表明,AOS 改变了 3681 个相关基因的表达,并确定了不同表达的转录因子群,突出了 AP-2、WRKY 和 NAC 的潜在参与。此外,还发现 bHLH13、bHLH17 和 URH2 是 JA 介导的花瓣衰老的潜在新调节因子。总之,我们的研究结果表明了一种新的遗传途径,通过这种途径,JA 可调节拟南芥花瓣的衰老。这一途径的运行独立于雄蕊的发育和叶片的衰老,表明花瓣衰老的专门机制正在进化。
ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis
Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in Arabidopsis was investigated. We showed that petal senescence in aos mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in dad1 and coi1 mutants. However, MYB21/24 and MYC2/3/4, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that AOS altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, bHLH13, bHLH17 and URH2 were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in Arabidopsis. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.
期刊介绍:
Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.