莼菜（莼菜科，叶绿体）模式标本的遗传分析解析了东北太平洋的叶片形成物种

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-08 DOI:10.1515/bot-2023-0072

Jeffery R. Hughey, Kathy Ann Miller, Paul W. Gabrielson

{"title":"莼菜（莼菜科，叶绿体）模式标本的遗传分析解析了东北太平洋的叶片形成物种","authors":"Jeffery R. Hughey, Kathy Ann Miller, Paul W. Gabrielson","doi":"10.1515/bot-2023-0072","DOIUrl":null,"url":null,"abstract":"Misapplication of Ulva epithets in GenBank has led to confusion in the scientific literature and community. To solve some of the problems, targeted DNA sequencing of plastid encoded rbcL gene amplicons or high-throughput sequencing was performed on all blade-forming Ulva type specimens from the northeast Pacific. Recently collected specimens from at or near type localities were also analyzed for some taxa. Based on these genetic analyses, we confirmed currently recognized species: U. californica, with U. angusta and U. scagelii as heterotypic synonyms, U. stenophylla, U. taeniata, and U. tanneri. Ulva dactylifera, currently considered a synonym of U. taeniata based on morpho-anatomy, is recognized as a distinct species, as is U. expansa whose type specimen was sequenced in 2018. All but two of the ITS, rbcL and tufA sequences in GenBank that were labeled U. californica were correctly named, in contrast to U. taeniata, for which only one of 14 sequences was correctly labeled. These results show that DNA sequencing of Ulva type specimens is essential for the correct application of names.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic analysis of Ulva (Ulvaceae, Chlorophyta) type specimens resolves northeast Pacific blade-forming species\",\"authors\":\"Jeffery R. Hughey, Kathy Ann Miller, Paul W. Gabrielson\",\"doi\":\"10.1515/bot-2023-0072\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Misapplication of Ulva epithets in GenBank has led to confusion in the scientific literature and community. To solve some of the problems, targeted DNA sequencing of plastid encoded rbcL gene amplicons or high-throughput sequencing was performed on all blade-forming Ulva type specimens from the northeast Pacific. Recently collected specimens from at or near type localities were also analyzed for some taxa. Based on these genetic analyses, we confirmed currently recognized species: U. californica, with U. angusta and U. scagelii as heterotypic synonyms, U. stenophylla, U. taeniata, and U. tanneri. Ulva dactylifera, currently considered a synonym of U. taeniata based on morpho-anatomy, is recognized as a distinct species, as is U. expansa whose type specimen was sequenced in 2018. All but two of the ITS, rbcL and tufA sequences in GenBank that were labeled U. californica were correctly named, in contrast to U. taeniata, for which only one of 14 sequences was correctly labeled. These results show that DNA sequencing of Ulva type specimens is essential for the correct application of names.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1515/bot-2023-0072\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1515/bot-2023-0072","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

GenBank 中对石莼名称的错误应用导致了科学文献和学术界的混乱。为了解决这些问题，我们对东北太平洋地区所有叶片型莼菜模式标本进行了质体编码的 rbcL 基因扩增子的定向 DNA 测序或高通量测序。此外，还分析了最近从类型产地或附近采集的标本中的一些分类群。根据这些基因分析，我们确认了目前公认的物种：angusta和 U. scagelii为异型异名；U. stenophylla、U. taeniata和 U. tanneri。根据形态解剖学，目前被认为是 U. taeniata 的异名的 Ulva dactylifera 被认定为一个独立的种，2018 年对其模式标本进行测序的 U. expansa 也被认定为一个独立的种。在 GenBank 中，除了两条 ITS、rbcL 和 tufA 序列外，所有被标注为加州乌贼的序列都被正确命名，而与之相反的是，14 条序列中只有一条被正确标注为加州乌贼（U. taeniata）。这些结果表明，莼菜模式标本的 DNA 测序对于正确应用名称至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Genetic analysis of Ulva (Ulvaceae, Chlorophyta) type specimens resolves northeast Pacific blade-forming species

Misapplication of Ulva epithets in GenBank has led to confusion in the scientific literature and community. To solve some of the problems, targeted DNA sequencing of plastid encoded rbcL gene amplicons or high-throughput sequencing was performed on all blade-forming Ulva type specimens from the northeast Pacific. Recently collected specimens from at or near type localities were also analyzed for some taxa. Based on these genetic analyses, we confirmed currently recognized species: U. californica, with U. angusta and U. scagelii as heterotypic synonyms, U. stenophylla, U. taeniata, and U. tanneri. Ulva dactylifera, currently considered a synonym of U. taeniata based on morpho-anatomy, is recognized as a distinct species, as is U. expansa whose type specimen was sequenced in 2018. All but two of the ITS, rbcL and tufA sequences in GenBank that were labeled U. californica were correctly named, in contrast to U. taeniata, for which only one of 14 sequences was correctly labeled. These results show that DNA sequencing of Ulva type specimens is essential for the correct application of names.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.