{"title":"在唇裂手术缺陷中应用亚叶酸和 A 型肉毒毒素的体内评估。","authors":"Atena Shiva, Parisa Soltani, Amirhossein Moaddabi, Rezvan Yazdian, Majid Saeedi, Zohreh Mozoun, Atefe Esfandpour, Carlo Rengo, Parastoo Namdar, Gianrico Spagnuolo","doi":"10.1111/ocr.12775","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Folinic acid and botulinum toxin A have shown promising results in wound healing in different studies. This study aimed to compare the effects of these approaches on wound healing after simulating cleft lip surgery in rats.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this experimental animal study, after creating lip defects, 30 rats were randomly divided into three groups and received normal saline (CTL), botulinum toxin A (BOT), and folinic acid (FOL). Biopsy from the skin wounds was performed after 14- and 28-days. These samples were stained with haematoxylin and eosin and Masson trichrome staining. Finally, each pathological parameter of wound healing was rated in this study.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>While the inflammatory response was not different among the study groups, fibroblast proliferation and collagen deposition were significantly higher in FOL group compared to BOT group. Moreover, both BOT and FOL facilitated epithelial healing and 14-day angiogenesis as compared with normal saline.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Improved wound healing was observed using both botulinum toxin A and folinic acid in rat animal models. However, the application of botulinum toxin A caused less fibroblast proliferation and collagen deposition which can potentially lead to less scar formation, which can be particularly important in the aesthetic zone.</p>\n </section>\n </div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In-vivo assessment of application of folinic acid and botulinum toxin A in cleft lip surgical defects\",\"authors\":\"Atena Shiva, Parisa Soltani, Amirhossein Moaddabi, Rezvan Yazdian, Majid Saeedi, Zohreh Mozoun, Atefe Esfandpour, Carlo Rengo, Parastoo Namdar, Gianrico Spagnuolo\",\"doi\":\"10.1111/ocr.12775\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>Folinic acid and botulinum toxin A have shown promising results in wound healing in different studies. This study aimed to compare the effects of these approaches on wound healing after simulating cleft lip surgery in rats.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this experimental animal study, after creating lip defects, 30 rats were randomly divided into three groups and received normal saline (CTL), botulinum toxin A (BOT), and folinic acid (FOL). Biopsy from the skin wounds was performed after 14- and 28-days. These samples were stained with haematoxylin and eosin and Masson trichrome staining. Finally, each pathological parameter of wound healing was rated in this study.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>While the inflammatory response was not different among the study groups, fibroblast proliferation and collagen deposition were significantly higher in FOL group compared to BOT group. Moreover, both BOT and FOL facilitated epithelial healing and 14-day angiogenesis as compared with normal saline.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Improved wound healing was observed using both botulinum toxin A and folinic acid in rat animal models. However, the application of botulinum toxin A caused less fibroblast proliferation and collagen deposition which can potentially lead to less scar formation, which can be particularly important in the aesthetic zone.</p>\\n </section>\\n </div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-03-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ocr.12775\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ocr.12775","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
简介在不同的研究中，亚叶酸和 A 型肉毒毒素在伤口愈合方面显示出良好的效果。本研究旨在比较这两种方法对模拟唇裂手术后大鼠伤口愈合的影响：在这项动物实验研究中，30 只大鼠在唇部缺损后被随机分为三组，分别接受生理盐水（CTL）、A 型肉毒毒素（BOT）和亚叶酸（FOL）治疗。分别在 14 天和 28 天后对皮肤伤口进行活检。这些样本用血红素和伊红以及马森三色染色法进行染色。最后，本研究对伤口愈合的各项病理参数进行了评分：结果：虽然各研究组的炎症反应没有差异，但 FOL 组的成纤维细胞增殖和胶原沉积明显高于 BOT 组。此外，与生理盐水相比，BOT 和 FOL 都能促进上皮愈合和 14 天的血管生成：结论：在大鼠动物模型中使用 A 型肉毒杆菌毒素和亚叶酸都能改善伤口愈合。然而，肉毒杆菌毒素 A 的应用导致的成纤维细胞增殖和胶原沉积较少，这可能会减少疤痕的形成，这对美容区尤为重要。
In-vivo assessment of application of folinic acid and botulinum toxin A in cleft lip surgical defects
Introduction
Folinic acid and botulinum toxin A have shown promising results in wound healing in different studies. This study aimed to compare the effects of these approaches on wound healing after simulating cleft lip surgery in rats.
Methods
In this experimental animal study, after creating lip defects, 30 rats were randomly divided into three groups and received normal saline (CTL), botulinum toxin A (BOT), and folinic acid (FOL). Biopsy from the skin wounds was performed after 14- and 28-days. These samples were stained with haematoxylin and eosin and Masson trichrome staining. Finally, each pathological parameter of wound healing was rated in this study.
Results
While the inflammatory response was not different among the study groups, fibroblast proliferation and collagen deposition were significantly higher in FOL group compared to BOT group. Moreover, both BOT and FOL facilitated epithelial healing and 14-day angiogenesis as compared with normal saline.
Conclusions
Improved wound healing was observed using both botulinum toxin A and folinic acid in rat animal models. However, the application of botulinum toxin A caused less fibroblast proliferation and collagen deposition which can potentially lead to less scar formation, which can be particularly important in the aesthetic zone.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
