{"title":"伊朗布什尔省海洋无脊椎动物（鳞目；无脊椎动物科）的抗阴道毛滴虫活性。","authors":"Hamideh Malekhayati, Afshar Bargahi, Soleiman Khorami, Mohsen Khataminejad, Moradali Fouladvand","doi":"10.4274/tpd.galenos.2023.96658","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The aim of the current research is to evaluate the antiparasite effects of compounds isolated from marine ascidian tunicates on <i>Trichomonas vaginalis</i>.</p><p><strong>Methods: </strong>Ascidian tunicates after collection were cut into small pieces, freeze-dried, and powdered. The resulting material was subjected to extraction in double-distilled water, ethanol, n-hexane, and dichloromethane. To fractionate the extracts and identify the most bioactive compound, silica gel column chromatography and GC-M/S analysis were used.</p><p><strong>Results: </strong>Fraction 18 of silica gel column chromatography of ethanol extract was the most effective against <i>T. vaginalis</i>. The respective IC<sub>50</sub>, CC<sub>50</sub>, and SI values for fraction 18 were 28.62 μg/mL, ˃800 μg/mL, and ˃27.95. GC-M/S analysis of this fraction identified a major phenolic compound (2, 4-bis (1, 1-dimethyl ethyl), whose toxicity against vero cells was only 10.15%.</p><p><strong>Conclusion: </strong>The ethanolic fraction containing phenol-2,4-bis (1,1-dimethylethyl), which has a potent lethality effect on <i>T. vaginalis</i>, may be considered as an antiparasite drug candidate.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 1","pages":"21-26"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-<i>Trichomonas vaginalis</i> Activity of Marine Ascidians (Tunicates; Ascidiacea) from the Bushehr Province, Iran\",\"authors\":\"Hamideh Malekhayati, Afshar Bargahi, Soleiman Khorami, Mohsen Khataminejad, Moradali Fouladvand\",\"doi\":\"10.4274/tpd.galenos.2023.96658\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The aim of the current research is to evaluate the antiparasite effects of compounds isolated from marine ascidian tunicates on <i>Trichomonas vaginalis</i>.</p><p><strong>Methods: </strong>Ascidian tunicates after collection were cut into small pieces, freeze-dried, and powdered. The resulting material was subjected to extraction in double-distilled water, ethanol, n-hexane, and dichloromethane. To fractionate the extracts and identify the most bioactive compound, silica gel column chromatography and GC-M/S analysis were used.</p><p><strong>Results: </strong>Fraction 18 of silica gel column chromatography of ethanol extract was the most effective against <i>T. vaginalis</i>. The respective IC<sub>50</sub>, CC<sub>50</sub>, and SI values for fraction 18 were 28.62 μg/mL, ˃800 μg/mL, and ˃27.95. GC-M/S analysis of this fraction identified a major phenolic compound (2, 4-bis (1, 1-dimethyl ethyl), whose toxicity against vero cells was only 10.15%.</p><p><strong>Conclusion: </strong>The ethanolic fraction containing phenol-2,4-bis (1,1-dimethylethyl), which has a potent lethality effect on <i>T. vaginalis</i>, may be considered as an antiparasite drug candidate.</p>\",\"PeriodicalId\":34974,\"journal\":{\"name\":\"Turkiye parazitolojii dergisi\",\"volume\":\"48 1\",\"pages\":\"21-26\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkiye parazitolojii dergisi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4274/tpd.galenos.2023.96658\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkiye parazitolojii dergisi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4274/tpd.galenos.2023.96658","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Anti-Trichomonas vaginalis Activity of Marine Ascidians (Tunicates; Ascidiacea) from the Bushehr Province, Iran
Objective: The aim of the current research is to evaluate the antiparasite effects of compounds isolated from marine ascidian tunicates on Trichomonas vaginalis.
Methods: Ascidian tunicates after collection were cut into small pieces, freeze-dried, and powdered. The resulting material was subjected to extraction in double-distilled water, ethanol, n-hexane, and dichloromethane. To fractionate the extracts and identify the most bioactive compound, silica gel column chromatography and GC-M/S analysis were used.
Results: Fraction 18 of silica gel column chromatography of ethanol extract was the most effective against T. vaginalis. The respective IC50, CC50, and SI values for fraction 18 were 28.62 μg/mL, ˃800 μg/mL, and ˃27.95. GC-M/S analysis of this fraction identified a major phenolic compound (2, 4-bis (1, 1-dimethyl ethyl), whose toxicity against vero cells was only 10.15%.
Conclusion: The ethanolic fraction containing phenol-2,4-bis (1,1-dimethylethyl), which has a potent lethality effect on T. vaginalis, may be considered as an antiparasite drug candidate.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
