来自人类沃顿果冻的间充质基质细胞在糖皮质激素高血压模型中调节眼内免疫反应:一项探索性分析。

IF 2 4区 医学 Q2 OPHTHALMOLOGY
Ophthalmic Research Pub Date : 2024-01-01 Epub Date: 2024-03-08 DOI:10.1159/000538183
Karine Dos Santos Evangelho, Carlos Cifuentes-González, William Rojas-Carabali, Clemencia De Vivero-Arciniegas, Mariana Cañas-Arboleda, Gustavo Salguero, Carolina Ramírez-Santana, Alejandra de-la-Torre
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引用次数: 0

摘要

导言青光眼是一种以视网膜神经节细胞丧失为特征的神经退行性疾病。最新研究表明,细胞因子失衡等免疫学变化可能在青光眼的病理生理学中发挥作用。这意味着免疫调节(类似于间质细胞产生的免疫调节)可能是治疗这种疾病的潜在途径。然而,在眼压过高(OH)模型中,尚未评估过玻璃体内注射人沃顿果冻衍生间充质基质细胞(hWJ-MSCs)对眼内免疫反应的影响:我们通过测量15只随机分群的新西兰兔的细胞因子水平和其他标记物(如神经胶质纤维酸性蛋白(GFAP)和T细胞)的表达进行了探讨:G1:OH;G2:hWJ-间充质干细胞;G3:OH+hWJ-间充质干细胞。我们使用 ELISA 和流式细胞术(细胞群)分析了房水(IL-6、IL-8 和 TNF-α)和玻璃体(IFN-γ、IL-10 和 TGF-β),并通过免疫组化分析了视网膜和视神经中的 TCD3+、TCD3+/TCD4+、TCD3+/TCD8+ 淋巴细胞和 GFAP:我们发现,与 G1 相比,G3 中 TNF-α、IL-6、IFN-γ、IL-10 和 IL-8 的含量有所下降,而 G2 和 G3 中 TGF-β 的含量均有所增加。所有组中的 TCD3+ 视网膜浸润主要是 TCD8+ 而不是 TCD4+ 细胞,所有组的视网膜和视神经中都观察到强烈的 GFAP 表达:我们的研究结果表明,细胞和体液免疫反应可能在青光眼视神经病变中发挥作用,而玻璃体内 hWJ-间充质干细胞可通过抑制促炎细胞因子和增强调节细胞因子来诱导免疫抑制环境。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mesenchymal Stromal Cells from Human Wharton's Jelly Modulate the Intraocular Immune Response in a Glucocorticoid Hypertension Model: An Exploratory Analysis.

Introduction: Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells. Recent research suggests immunological changes such as cytokine imbalance may affect its pathophysiology. This implies that immunomodulation, like that of mesenchymal cells, could be a potential therapeutic avenue for this disease. However, the effects of intravitreal injections of human Wharton's jelly-derived mesenchymal stromal cells (hWJ-MSCs) on intraocular immune response have not been assessed in ocular hypertension (OH) models.

Methods: We explored this by measuring cytokine levels and expression of other markers, such as glial fibrillary acidic protein (GFAP) and T cells, in 15 randomly divided New Zealand rabbits: G1: OH, G2: hWJ-MSCs, and G3: OH+hWJ-MSCs. We analyzed the aqueous humor (IL-6, IL-8, and TNF-α) and vitreous humor (IFN-γ, IL-10, and TGF-β) using ELISA and flow cytometry (cell populations), as well as TCD3+, TCD3+/TCD4+, and TCD3+/TCD8+ lymphocytes, and GFAP in the retina and optic nerve through immunohistochemistry.

Results: We found a decrease in TNF-α, IL-6, IFN-γ, IL-10, and IL-8 in G3 compared to G1 and an increase in TGF-β in both G2 and G3. TCD3+ retinal infiltration in all groups was primarily TCD8+ rather than TCD4+ cells, and strong GFAP expression was observed in both the retina and optic nerves in all groups.

Conclusion: Our results suggest that cellular and humoral immune responses may play a role in glaucomatous optic neuropathy and that intravitreal hWJ-MSCs can induce an immunosuppressive environment by inhibiting proinflammatory cytokines and enhancing regulatory cytokines.

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来源期刊
Ophthalmic Research
Ophthalmic Research 医学-眼科学
CiteScore
3.80
自引率
4.80%
发文量
75
审稿时长
6-12 weeks
期刊介绍: ''Ophthalmic Research'' features original papers and reviews reporting on translational and clinical studies. Authors from throughout the world cover research topics on every field in connection with physical, physiologic, pharmacological, biochemical and molecular biological aspects of ophthalmology. This journal also aims to provide a record of international clinical research for both researchers and clinicians in ophthalmology. Finally, the transfer of information from fundamental research to clinical research and clinical practice is particularly welcome.
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