{"title":"新型氢氧化钙生物可降解纳米颗粒作为龋内药物的抗骨质疏松活性评价。","authors":"Patarawadee Promta DDS , Patcharaporn Chaiyosang DDS , Aussara Panya PhD , Pongrapee Laorodphun MS , Warat Leelapornpisid DDS, PhD , Napatsorn Imerb DDS, PhD","doi":"10.1016/j.joen.2024.02.023","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>The aim of this study was to evaluate the anti-osteoclastic activity of calcium hydroxide–loaded poly(lactic-co-glycolic acid) nanoparticles [Ca(OH)<sub>2</sub>-loaded PLGA NPs] in comparison to calcium hydroxide nanoparticles [Ca(OH)<sub>2</sub> NPs].</p></div><div><h3>Methods</h3><p>RAW 264.7 cell lines (third–fifth passage) were cultured and incubated with soluble receptor activator of nuclear factor kappa B ligand in triplicate. Subsequently, Ca(OH)<sub>2</sub>-loaded PLGA NPs and Ca(OH)<sub>2</sub> NPs were added for 7 days to evaluate their effects on receptor activator of nuclear factor kappa B ligand–induced osteoclast differentiation of RAW 264.7 cells by tartrate-resistant acid phosphatase activity. Additionally, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to confirm the cytotoxicity of treatments to cells.</p></div><div><h3>Results</h3><p>Tartrate-resistant acid phosphatase staining showed a significant reduction in the osteoclast number when treated with Ca(OH)<sub>2</sub>-loaded PLGA NPs compared with Ca(OH)<sub>2</sub> NPs (<em>P</em> < .01). In comparison to the control, the number of osteoclasts significantly reduced upon treatment with Ca(OH)<sub>2</sub>-loaded PLGA NPs (<em>P</em> < .05), but there was no significant difference in Ca(OH)<sub>2</sub> NPs. Furthermore, osteoclast morphology in both treatment groups exhibited smaller sizes than the control group. Neither Ca(OH)<sub>2</sub>-loaded PLGA NPs nor Ca(OH)<sub>2</sub> NPs demonstrated cytotoxic effects on RAW264.7 cells.</p></div><div><h3>Conclusions</h3><p>Both Ca(OH)<sub>2</sub> NPs with and without poly(lactic-co-glycolic acid) have the ability to inhibit osteoclast differentiation. However, Ca(OH)<sub>2</sub>-loaded PLGA NPs exhibit greater potential than Ca(OH)<sub>2</sub> NPs, making them a promising intracanal medicament for cases of root resorption.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Evaluation of Anti-Osteoclastic Activity of the Novel Calcium Hydroxide Biodegradable Nanoparticles as an Intracanal Medicament\",\"authors\":\"Patarawadee Promta DDS , Patcharaporn Chaiyosang DDS , Aussara Panya PhD , Pongrapee Laorodphun MS , Warat Leelapornpisid DDS, PhD , Napatsorn Imerb DDS, PhD\",\"doi\":\"10.1016/j.joen.2024.02.023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>The aim of this study was to evaluate the anti-osteoclastic activity of calcium hydroxide–loaded poly(lactic-co-glycolic acid) nanoparticles [Ca(OH)<sub>2</sub>-loaded PLGA NPs] in comparison to calcium hydroxide nanoparticles [Ca(OH)<sub>2</sub> NPs].</p></div><div><h3>Methods</h3><p>RAW 264.7 cell lines (third–fifth passage) were cultured and incubated with soluble receptor activator of nuclear factor kappa B ligand in triplicate. Subsequently, Ca(OH)<sub>2</sub>-loaded PLGA NPs and Ca(OH)<sub>2</sub> NPs were added for 7 days to evaluate their effects on receptor activator of nuclear factor kappa B ligand–induced osteoclast differentiation of RAW 264.7 cells by tartrate-resistant acid phosphatase activity. Additionally, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to confirm the cytotoxicity of treatments to cells.</p></div><div><h3>Results</h3><p>Tartrate-resistant acid phosphatase staining showed a significant reduction in the osteoclast number when treated with Ca(OH)<sub>2</sub>-loaded PLGA NPs compared with Ca(OH)<sub>2</sub> NPs (<em>P</em> < .01). In comparison to the control, the number of osteoclasts significantly reduced upon treatment with Ca(OH)<sub>2</sub>-loaded PLGA NPs (<em>P</em> < .05), but there was no significant difference in Ca(OH)<sub>2</sub> NPs. Furthermore, osteoclast morphology in both treatment groups exhibited smaller sizes than the control group. Neither Ca(OH)<sub>2</sub>-loaded PLGA NPs nor Ca(OH)<sub>2</sub> NPs demonstrated cytotoxic effects on RAW264.7 cells.</p></div><div><h3>Conclusions</h3><p>Both Ca(OH)<sub>2</sub> NPs with and without poly(lactic-co-glycolic acid) have the ability to inhibit osteoclast differentiation. However, Ca(OH)<sub>2</sub>-loaded PLGA NPs exhibit greater potential than Ca(OH)<sub>2</sub> NPs, making them a promising intracanal medicament for cases of root resorption.</p></div>\",\"PeriodicalId\":15703,\"journal\":{\"name\":\"Journal of endodontics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of endodontics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0099239924001560\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of endodontics","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0099239924001560","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
The Evaluation of Anti-Osteoclastic Activity of the Novel Calcium Hydroxide Biodegradable Nanoparticles as an Intracanal Medicament
Introduction
The aim of this study was to evaluate the anti-osteoclastic activity of calcium hydroxide–loaded poly(lactic-co-glycolic acid) nanoparticles [Ca(OH)2-loaded PLGA NPs] in comparison to calcium hydroxide nanoparticles [Ca(OH)2 NPs].
Methods
RAW 264.7 cell lines (third–fifth passage) were cultured and incubated with soluble receptor activator of nuclear factor kappa B ligand in triplicate. Subsequently, Ca(OH)2-loaded PLGA NPs and Ca(OH)2 NPs were added for 7 days to evaluate their effects on receptor activator of nuclear factor kappa B ligand–induced osteoclast differentiation of RAW 264.7 cells by tartrate-resistant acid phosphatase activity. Additionally, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to confirm the cytotoxicity of treatments to cells.
Results
Tartrate-resistant acid phosphatase staining showed a significant reduction in the osteoclast number when treated with Ca(OH)2-loaded PLGA NPs compared with Ca(OH)2 NPs (P < .01). In comparison to the control, the number of osteoclasts significantly reduced upon treatment with Ca(OH)2-loaded PLGA NPs (P < .05), but there was no significant difference in Ca(OH)2 NPs. Furthermore, osteoclast morphology in both treatment groups exhibited smaller sizes than the control group. Neither Ca(OH)2-loaded PLGA NPs nor Ca(OH)2 NPs demonstrated cytotoxic effects on RAW264.7 cells.
Conclusions
Both Ca(OH)2 NPs with and without poly(lactic-co-glycolic acid) have the ability to inhibit osteoclast differentiation. However, Ca(OH)2-loaded PLGA NPs exhibit greater potential than Ca(OH)2 NPs, making them a promising intracanal medicament for cases of root resorption.
期刊介绍:
The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.