西洋扁虱丝氨酸/苏氨酸激酶 AKT 和磷酸肌醇依赖性激酶 PDK 对三种食物的反应及其对繁殖功能的调控

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xinyue Qiu, Wanqing Huang, Wenbo Yue, Dingyin Li, Junrui Zhi
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引用次数: 0

摘要

Frankliniella occidentalis (Pergande) 是一种典型的杂食性昆虫,以寄主植物、花粉和螨卵为食,对全球农作物构成威胁。胰岛素信号通路(ISP)是一种典型的营养敏感通路,参与调节昆虫的各种功能。丝氨酸/苏氨酸激酶(AKTs)和磷酸肌醇依赖性激酶(PDKs)是 ISP 的关键组成部分。本研究克隆了西黄花蝇的 FoAKT 和 FoPDK 基因,并测定了三种食物对其表达的影响。以芸豆叶为饲料并辅以松花粉或螨卵的蓟马,其 FoAKT 和 FoPDK 的表达量高于仅以芸豆叶为饲料的蓟马。同时,以添加松花粉的芸豆叶为食的蓟马繁殖力最高。此外,RNA 干扰介导的 FoAKT 和 FoPDK 基因敲除会降低雌虫体内卵黄素(Vg)含量和 Vg 表达,缩短卵节长度,延迟卵子发育,降低受精率和后代孵化率。此外,幼体激素(JH)的合成减少,葡萄糖、三卤糖、糖原和三卤酶的含量也受到影响。这些结果表明,FoAKT 和 FoPDK 可通过调节 Vg 和 JH 的产生以及碳水化合物的代谢来调控闭壳龟的繁殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Response of the serine/threonine kinase AKT and phosphoinositide-dependent kinase PDK in Frankliniella occidentalis (Thysanoptera: Thripidae) to three kinds of foods and their regulation of reproductive function

Response of the serine/threonine kinase AKT and phosphoinositide-dependent kinase PDK in Frankliniella occidentalis (Thysanoptera: Thripidae) to three kinds of foods and their regulation of reproductive function

Frankliniella occidentalis (Pergande) is a typical omnivorous insect that feeds on host plants, pollens and mite eggs, and poses a threat to crops worldwide. The insulin signalling pathway (ISP) is a typical nutrient-sensitive pathway that participates in the regulation of various functions in insects. Serine/threonine kinases (AKTs) and phosphoinositide-dependent kinases (PDKs) are key components of the ISP. In this study, the FoAKT and FoPDK genes in F. occidentalis were cloned, and the effects of three foods on their expression were determined. The expression of FoAKT and FoPDK in the thrips fed on kidney bean leaves supplemented with pine pollen or mite eggs was higher than in those primarily fed on leaves alone. Meanwhile, the fecundity of thrips fed on leaves supplemented with pine pollen was highest. In addition, RNA interference-mediated knockdown of FoAKT and FoPDK decreased vitellogenin (Vg) content and Vg expression in females, shortened ovariole length, delayed egg development and reduced fecundity and offspring hatching rates. Furthermore, the synthesis of juvenile hormone (JH) was reduced, and the contents of glucose, trehalose, glycogen and trehalase were affected. These results suggest that FoAKT and FoPDK regulate the reproduction of F. occidentalis by regulating Vg and JH production as well as carbohydrate metabolism.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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