RNA 引导的基因组工程：向转座子的范式转变。

IF 14.3 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Trends in biotechnology Pub Date : 2024-08-01 Epub Date: 2024-03-04 DOI:10.1016/j.tibtech.2024.02.006

Chin-Wei Chang, Vy Anh Truong, Nam Ngoc Pham, Yu-Chen Hu

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引用次数: 0

摘要

CRISPR-Cas 系统为基因组工程领域带来了革命性的变化，但它需要诱导双链断裂（DSB），而且由于其蛋白质体积较大，可能难以输送。Tn7类转座子（如CRISPR相关转座子（CAST））可重新用于RNA引导的无DSB整合，而IS200/IS605转座子家族的强制性移动元件引导活动（OMEGA）蛋白已被开发为超小型RNA引导的基因组编辑工具。CASTs 和 OMEGA 是令人兴奋的创新基因组工程工具，可以提高编辑的精度和效率。本综述探讨了 CASTs 和 OMEGA 在原核细胞和真核细胞基因组编辑中的最新发展和应用。与其他 CRISPR 系统相比，本综述探讨了这些基于转座子的系统的优缺点。

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RNA-guided genome engineering: paradigm shift towards transposons.

CRISPR-Cas systems revolutionized the genome engineering field but need to induce double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size. Tn7-like transposons such as CRISPR-associated transposons (CASTs) can be repurposed for RNA-guided DSB-free integration, and obligate mobile element guided activity (OMEGA) proteins of the IS200/IS605 transposon family have been developed as hypercompact RNA-guided genome editing tools. CASTs and OMEGA are exciting, innovative genome engineering tools that can improve the precision and efficiency of editing. This review explores the recent developments and uses of CASTs and OMEGA in genome editing across prokaryotic and eukaryotic cells. The pros and cons of these transposon-based systems are deliberated in comparison to other CRISPR systems.

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来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).