Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam
{"title":"负载纳米铋颗粒和氯化十六烷基吡啶的藻酸盐膜的抗菌和抗肿瘤活性。","authors":"Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam","doi":"10.1177/22808000241236590","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.</p><p><strong>Material and methods: </strong>The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.</p><p><strong>Results: </strong>After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of <i>K. pneumoniae</i> by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of <i>Salmonella</i>, <i>E. faecalis</i> and <i>E. faecium</i> by 82.9%, 82.6%, and 78%, respectively (<i>p < 0.0001</i>). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for <i>K. pneumoniae, Salmonella, E. faecalis</i>, and <i>E. faecium</i>, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (<i>p < 0.0001</i>). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for <i>K. pneumoniae, E. faecium, E. faecalis</i>, and <i>Salmonella</i>, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.</p><p><strong>Conclusion: </strong>An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. Therefore, it could be applied in cancer treatment and to diminish the occurrence of surgical site infections.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride.\",\"authors\":\"Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam\",\"doi\":\"10.1177/22808000241236590\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.</p><p><strong>Material and methods: </strong>The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.</p><p><strong>Results: </strong>After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of <i>K. pneumoniae</i> by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of <i>Salmonella</i>, <i>E. faecalis</i> and <i>E. faecium</i> by 82.9%, 82.6%, and 78%, respectively (<i>p < 0.0001</i>). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for <i>K. pneumoniae, Salmonella, E. faecalis</i>, and <i>E. faecium</i>, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (<i>p < 0.0001</i>). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for <i>K. pneumoniae, E. faecium, E. faecalis</i>, and <i>Salmonella</i>, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.</p><p><strong>Conclusion: </strong>An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. 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Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride.
Objective: To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.
Material and methods: The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.
Results: After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of K. pneumoniae by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of Salmonella, E. faecalis and E. faecium by 82.9%, 82.6%, and 78%, respectively (p < 0.0001). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for K. pneumoniae, Salmonella, E. faecalis, and E. faecium, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (p < 0.0001). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for K. pneumoniae, E. faecium, E. faecalis, and Salmonella, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.
Conclusion: An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. Therefore, it could be applied in cancer treatment and to diminish the occurrence of surgical site infections.
期刊介绍：
The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials.
The areas covered by the journal will include:
• Biomaterials / Materials for biomedical applications
• Functional materials
• Hybrid and composite materials
• Soft materials
• Hydrogels
• Nanomaterials
• Gene delivery
• Nonodevices
• Metamaterials
• Active coatings
• Surface functionalization
• Tissue engineering
• Cell delivery/cell encapsulation systems
• 3D printing materials
• Material characterization
• Biomechanics
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