基于基因组学的四种猪源性乳酸菌株分析及其作为潜在益生菌的评估。

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Dekai Niu, Ni Feng, Siteng Xi, Jianjian Xu, Yong Su
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引用次数: 0

摘要

寻找益生菌并探索其功能对畜牧业至关重要。最近,猪源性乳酸菌(LAB)显示出作为益生菌的巨大潜力。然而,通过基因组学分析评估猪源性乳酸菌作为潜在益生菌的研究相对有限。本研究利用基因组学技术分析了四种猪源性 LAB 菌株(约翰逊乳杆菌 L16、卷曲乳杆菌 ZHA1、唾液乳杆菌 ZSA5 和动物乳杆菌 ZSB1),并结合体外试验评估了它们作为益生菌的潜力。这四种菌株的基因组大小从 1,897,301 bp 到 2,318,470 bp 不等,GC 含量从 33.03% 到 41.97%。泛基因组分析和共线性分析表明,四株菌株的基因组之间存在差异。碳水化合物活性酶分析表明,约翰逊酵母 L16 编码的碳水化合物活性酶多于其他菌株。KEGG 通路分析和体外试验证实,L. johnsonii L16 可以利用多种碳水化合物,并且对每种碳水化合物都有很好的利用能力。这四种菌株都含有与耐酸性相关的基因,并能耐受低 pH 值,其中 L. johnsonii L16 的耐酸性最强。四株菌株含有与胆盐耐受性相关的基因,能够耐受 0.1% 的胆盐。四株菌株含有抗氧化相关基因,并在体外测试中表现出抗氧化活性。它们含有与有机酸生物合成相关的基因,对肠毒素性大肠杆菌 K88(ETEC K88)和沙门氏菌 6,7:c:1,5具有抗菌活性,其中约翰逊酵母菌 L16 和唾液酵母菌 ZSA5 具有编码细菌素的基因簇。结果表明,基因组分析与体外测试相结合是评估不同菌株作为益生菌的有效方法。本研究结果表明,在四种菌株中,L. johnsonii L16具有作为益生菌株的潜力,并为猪生产中益生菌的开发提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genomics-based analysis of four porcine-derived lactic acid bacteria strains and their evaluation as potential probiotics.

Genomics-based analysis of four porcine-derived lactic acid bacteria strains and their evaluation as potential probiotics.

The search for probiotics and exploration of their functions are crucial for livestock farming. Recently, porcine-derived lactic acid bacteria (LAB) have shown great potential as probiotics. However, research on the evaluation of porcine-derived LAB as potential probiotics through genomics-based analysis is relatively limited. The present study analyzed four porcine-derived LAB strains (Lactobacillus johnsonii L16, Latilactobacillus curvatus ZHA1, Ligilactobacillus salivarius ZSA5 and Ligilactobacillus animalis ZSB1) using genomic techniques and combined with in vitro tests to evaluate their potential as probiotics. The genome sizes of the four strains ranged from 1,897,301 bp to 2,318,470 bp with the GC contents from 33.03 to 41.97%. Pan-genomic analysis and collinearity analysis indicated differences among the genomes of four strains. Carbohydrate active enzymes analysis revealed that L. johnsonii L16 encoded more carbohydrate active enzymes than other strains. KEGG pathway analysis and in vitro tests confirmed that L. johnsonii L16 could utilize a wide range of carbohydrates and had good utilization capacity for each carbohydrate. The four strains had genes related to acid tolerance and were tolerant to low pH, with L. johnsonii L16 showing the greatest tolerance. The four strains contained genes related to bile salt tolerance and were able to tolerate 0.1% bile salt. Four strains had antioxidant related genes and exhibited antioxidant activity in in vitro tests. They contained the genes linked with organic acid biosynthesis and exhibited antibacterial activity against enterotoxigenic Escherichia coli K88 (ETEC K88) and Salmonella 6,7:c:1,5, wherein, L. johnsonii L16 and L. salivarius ZSA5 had gene clusters encoding bacteriocin. Results suggest that genome analysis combined with in vitro tests is an effective approach for evaluating different strains as probiotics. The findings of this study indicate that L. johnsonii L16 has the potential as a probiotic strain among the four strains and provide theoretical basis for the development of probiotics in swine production.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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