Shan Chen, Xinghong Luo, Liu Yang, Liang Luo, Zhen Hu, Jianglan Wang
{"title":"藏红花酸通过激活自噬保护创伤性脑损伤模型中的小鼠大脑免受凋亡。","authors":"Shan Chen, Xinghong Luo, Liu Yang, Liang Luo, Zhen Hu, Jianglan Wang","doi":"10.1080/02699052.2024.2324022","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.</p><p><strong>Methods: </strong>We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.</p><p><strong>Results: </strong>Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.</p><p><strong>Conclusion: </strong>Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.</p>","PeriodicalId":9082,"journal":{"name":"Brain injury","volume":" ","pages":"524-530"},"PeriodicalIF":1.5000,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Crocetin protects mouse brain from apoptosis in traumatic brain injury model through activation of autophagy.\",\"authors\":\"Shan Chen, Xinghong Luo, Liu Yang, Liang Luo, Zhen Hu, Jianglan Wang\",\"doi\":\"10.1080/02699052.2024.2324022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.</p><p><strong>Methods: </strong>We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.</p><p><strong>Results: </strong>Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.</p><p><strong>Conclusion: </strong>Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.</p>\",\"PeriodicalId\":9082,\"journal\":{\"name\":\"Brain injury\",\"volume\":\" \",\"pages\":\"524-530\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brain injury\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02699052.2024.2324022\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain injury","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02699052.2024.2324022","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/3 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
Crocetin protects mouse brain from apoptosis in traumatic brain injury model through activation of autophagy.
Background: Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.
Methods: We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.
Results: Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.
Conclusion: Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.
期刊介绍：
Brain Injury publishes critical information relating to research and clinical practice, adult and pediatric populations. The journal covers a full range of relevant topics relating to clinical, translational, and basic science research. Manuscripts address emergency and acute medical care, acute and post-acute rehabilitation, family and vocational issues, and long-term supports. Coverage includes assessment and interventions for functional, communication, neurological and psychological disorders.
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