{"title":"开发对 pH 值敏感的阿仑膦酸盐装饰蚕丝纤维素/海藻酸盐纳米粒子,用于将多柔比星主动靶向治疗骨癌","authors":"Fatemeh Vafapour, Fatemeh Bagheri, Mehdi Farokhi","doi":"10.1007/s10876-024-02593-1","DOIUrl":null,"url":null,"abstract":"<p>Targeted drug delivery has a positive impact on the concentration of anticancer drugs at the tumor site and can reduce harmful side effects. In this research, silk fibroin and sodium alginate were utilized to produce nanoparticles, which were then targeted using sodium alendronate (SF-SA-ALN NPs) to improve the effectiveness of chemotherapy on osteosarcoma. The resulting nanoparticles had a surface charge of -50.5 mV and an average size of 252 nm (with PDI = 0.157). Doxorubicin (DOX) was loaded onto the nanoparticles using ionic interaction with 96% drug loading efficiency. The nanoparticles showed higher drug release at pH 5.4 compared to pH 7.4. It was also investigated the effectiveness of in vitro bone targeting using hydroxyapatite (HA) as a bone model, and found that targeted nanoparticles accumulated in HA crystals. The toxicity of the drug-loaded nanoparticles on a human osteosarcoma cell line (MG-63) revealed that the drug-targeted nanoparticles were more toxic than free drug after 24 h. This may be due to the presence of alendronate on the SF-SA-ALN NPs, which allowed for internalization of the nanoparticles through endocytosis. Overall, these nanoparticles show promise as a potential drug delivery system for osteosarcoma treatment.</p>","PeriodicalId":618,"journal":{"name":"Journal of Cluster Science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of pH-Sensitive Alendronate-Decorated Silk Fibroin/ Alginate Nanoparticles for Active Targeting of Doxorubicin to Bone Cancers\",\"authors\":\"Fatemeh Vafapour, Fatemeh Bagheri, Mehdi Farokhi\",\"doi\":\"10.1007/s10876-024-02593-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Targeted drug delivery has a positive impact on the concentration of anticancer drugs at the tumor site and can reduce harmful side effects. In this research, silk fibroin and sodium alginate were utilized to produce nanoparticles, which were then targeted using sodium alendronate (SF-SA-ALN NPs) to improve the effectiveness of chemotherapy on osteosarcoma. The resulting nanoparticles had a surface charge of -50.5 mV and an average size of 252 nm (with PDI = 0.157). Doxorubicin (DOX) was loaded onto the nanoparticles using ionic interaction with 96% drug loading efficiency. The nanoparticles showed higher drug release at pH 5.4 compared to pH 7.4. It was also investigated the effectiveness of in vitro bone targeting using hydroxyapatite (HA) as a bone model, and found that targeted nanoparticles accumulated in HA crystals. The toxicity of the drug-loaded nanoparticles on a human osteosarcoma cell line (MG-63) revealed that the drug-targeted nanoparticles were more toxic than free drug after 24 h. This may be due to the presence of alendronate on the SF-SA-ALN NPs, which allowed for internalization of the nanoparticles through endocytosis. Overall, these nanoparticles show promise as a potential drug delivery system for osteosarcoma treatment.</p>\",\"PeriodicalId\":618,\"journal\":{\"name\":\"Journal of Cluster Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cluster Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s10876-024-02593-1\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cluster Science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10876-024-02593-1","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Development of pH-Sensitive Alendronate-Decorated Silk Fibroin/ Alginate Nanoparticles for Active Targeting of Doxorubicin to Bone Cancers
Targeted drug delivery has a positive impact on the concentration of anticancer drugs at the tumor site and can reduce harmful side effects. In this research, silk fibroin and sodium alginate were utilized to produce nanoparticles, which were then targeted using sodium alendronate (SF-SA-ALN NPs) to improve the effectiveness of chemotherapy on osteosarcoma. The resulting nanoparticles had a surface charge of -50.5 mV and an average size of 252 nm (with PDI = 0.157). Doxorubicin (DOX) was loaded onto the nanoparticles using ionic interaction with 96% drug loading efficiency. The nanoparticles showed higher drug release at pH 5.4 compared to pH 7.4. It was also investigated the effectiveness of in vitro bone targeting using hydroxyapatite (HA) as a bone model, and found that targeted nanoparticles accumulated in HA crystals. The toxicity of the drug-loaded nanoparticles on a human osteosarcoma cell line (MG-63) revealed that the drug-targeted nanoparticles were more toxic than free drug after 24 h. This may be due to the presence of alendronate on the SF-SA-ALN NPs, which allowed for internalization of the nanoparticles through endocytosis. Overall, these nanoparticles show promise as a potential drug delivery system for osteosarcoma treatment.
期刊介绍:
The journal publishes the following types of papers: (a) original and important research;
(b) authoritative comprehensive reviews or short overviews of topics of current
interest; (c) brief but urgent communications on new significant research; and (d)
commentaries intended to foster the exchange of innovative or provocative ideas, and
to encourage dialogue, amongst researchers working in different cluster
disciplines.