Lumos maxima - 荧光团如何抵御光漂白?

IF 6.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuan Zhang , Jing Ling , Tianyan Liu , Zhixing Chen
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引用次数: 0

摘要

荧光染料与先进的显微镜技术相辅相成,使研究人员能够以较高的空间和时间分辨率研究生物大分子的位置和动态过程。然而,荧光染料的不稳定性,包括光漂白和光电转换,是超分辨率和延时成像的基本限制。在本综述中,我们将讨论提高荧光染料光稳定性的最新进展。我们总结了氰基和罗丹明染料的主要光漂白过程,并重点介绍了近年来为增强这些荧光团而开发的一系列策略。此外,我们还讨论了蛋白质微环境和标记方法对荧光团光稳定性的影响。我们的目标是激发下一代坚固而明亮的荧光团,最终实现活细胞超分辨率延时成像的常规实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lumos maxima – How robust fluorophores resist photobleaching?

Fluorescent dyes synergize with advanced microscopy for researchers to investigate the location and dynamic processes of biomacromolecules with high spatial and temporal resolution. However, the instability of fluorescent dyes, including photobleaching and photoconversion, represent fundamental limits for super-resolution and time-lapse imaging. In this review, we discuss the latest advances in improving the photostability of fluorescent dyes. We summarize the primary photobleaching processes of cyanine and rhodamine dyes and highlight a range of strategies developed in recent years to strengthen these fluorophores. Additionally, we discuss the influence of protein microenvironments and labeling methods on the photostability of fluorophores. We aim to inspire next-generation robust and bright fluorophores that ultimately enable the routine practice of time-lapse super-resolution imaging of live cells.

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来源期刊
Current Opinion in Chemical Biology
Current Opinion in Chemical Biology 生物-生化与分子生物学
CiteScore
13.30
自引率
1.30%
发文量
113
审稿时长
74 days
期刊介绍: COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.
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