CRISPR/Cas9 介导的对车轴草 ZjEIN2 基因的高效基因编辑

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jin Hee Kim, Mi-Young Park, Lanshuo Wang, Phan Phuong Thao Doan, Yueyue Yuan, Hyo-Yeon Lee, Jeongsik Kim
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引用次数: 0

摘要

基于 CRISPR/Cas9 的靶向基因编辑技术已成为破译基因功能和改良植物性状的多功能工具。然而,这种技术尚未应用于广泛用于绿地的著名暖季型草坪--紫云英(Zoysia japonica)。叶片衰老是影响作物质量的一个重要过程,它发生在 Z. japonica 的生长后期,降低了其美学价值和性能。在这项研究中,我们巧妙地利用 CRISPR/Cas9 介导的基因编辑技术,通过靶向 ZjEIN2 基因(乙烯介导的衰老的关键调控因子),创造出具有延迟叶片衰老的 Z. japonica。精确的基因编辑产生了 ZjEIN2 基因的敲除突变,从而导致在黑暗和乙烯处理条件下叶片衰老延迟。这为 ZjEIN2 在叶片衰老调控中的作用提供了有力证据。这些发现凸显了 CRISPR/Cas9 介导的基因编辑作为一种生物技术策略来增强粳稻及其他作物抗衰老性状的潜力。这项研究对可持续农业和草坪管理具有重要意义,为未来的应用和研究提供了广阔的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Efficient CRISPR/Cas9-mediated gene editing of the ZjEIN2 gene in Zoysia japonica

Efficient CRISPR/Cas9-mediated gene editing of the ZjEIN2 gene in Zoysia japonica

CRISPR/Cas9-based targeted gene editing has emerged as a versatile tool for deciphering gene function and improving traits in plants. However, this technique has not been applied to Zoysia japonica, a prominent warm-season turfgrass widely used for green spaces. Leaf senescence, a vital process affecting crop quality, occurs in Z. japonica during late growth, diminishing its aesthetic value and performance. In this study, we adeptly employed CRISPR/Cas9-mediated gene editing to create Z. japonica exhibiting delayed leaf senescence by targeting the ZjEIN2 gene, a crucial regulator of ethylene-mediated senescence. Precise gene editing, which generated knockout mutations in ZjEIN2, led to delayed leaf senescence in both dark and ethylene treatment conditions. This provided strong evidence for ZjEIN2’s role in leaf senescence regulation. These findings highlight the potential of CRISPR/Cas9-mediated gene editing as a biotechnological strategy to enhance anti-senescence traits in Z. japonica and potentially other crops. This study carries significant implications for sustainable agriculture and turfgrass management, offering promising avenues for future applications and research.

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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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