{"title":"花青素氰苷-3-O-葡萄糖苷的抗糖尿病作用:来自胰岛素抵抗肝细胞和糖尿病小鼠的数据","authors":"Xiang Ye, Wen Chen, Xu-Fan Huang, Fu-Jie Yan, Shui-Guang Deng, Xiao-Dong Zheng, Peng-Fei Shan","doi":"10.1038/s41387-024-00265-7","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3><p>Anthocyanins are a group of natural products widely found in plants. They have been found to alleviate the disorders of glucose metabolism in type 2 diabetes mellitus (T2DM), while the underlying mechanisms remain unclear.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>HepG2 and L02 cells were incubated with 0.2 mM PA and 30 mM glucose for 24 h to induce IR, and cells treated with 5 mM glucose were used as the control. C57BL/6 J male mice and db/db male mice were fed with a chow diet and gavaged with pure water or cyanidin-3-O-glucoside (C3G) solution (150 mg/kg/day) for 6 weeks.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>In this study, the anthocyanin C3G, extracted from red bayberry, was found to alleviate disorders of glucose metabolism, which resulted in increased insulin sensitivity in hepatocytes, and achieved by enhancing the glucose consumption as well as glycogen synthesis in insulin resistance (IR) hepatpcytes. Subsequently, the expression of key proteins involved in IR was detected by western blotting analysis. Protein tyrosine phosphatase-1B (PTP1B), a negative regulator of insulin signaling, could reduce cellular sensitivity to insulin by inhibiting the phosphorylation of insulin receptor substrate-2 (IRS-2). Results of this study showed that C3G inhibited the increase in PTP1B after high glucose and palmitic acid treatment. And this inhibition was accompanied by increased phosphorylation of IRS proteins. Furthermore, the effect of C3G on improving IR in vivo was validated by using a diabetic db/db mouse model.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>These findings demonstrated that C3G could alleviate IR in vitro and in vivo to increase insulin sensitivity, which may offer a new insight for regulating glucose metabolism during T2DM by using the natural dietary bioactive components.</p><figure><p>C3G promotes the phosphorylation of IRS-2 proteins by suppressing the expression of PTP1B, and then enhances the sensitivity of hepatocyte to insulin.</p></figure>","PeriodicalId":19339,"journal":{"name":"Nutrition & Diabetes","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-diabetic effect of anthocyanin cyanidin-3-O-glucoside: data from insulin resistant hepatocyte and diabetic mouse\",\"authors\":\"Xiang Ye, Wen Chen, Xu-Fan Huang, Fu-Jie Yan, Shui-Guang Deng, Xiao-Dong Zheng, Peng-Fei Shan\",\"doi\":\"10.1038/s41387-024-00265-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background</h3><p>Anthocyanins are a group of natural products widely found in plants. They have been found to alleviate the disorders of glucose metabolism in type 2 diabetes mellitus (T2DM), while the underlying mechanisms remain unclear.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>HepG2 and L02 cells were incubated with 0.2 mM PA and 30 mM glucose for 24 h to induce IR, and cells treated with 5 mM glucose were used as the control. C57BL/6 J male mice and db/db male mice were fed with a chow diet and gavaged with pure water or cyanidin-3-O-glucoside (C3G) solution (150 mg/kg/day) for 6 weeks.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>In this study, the anthocyanin C3G, extracted from red bayberry, was found to alleviate disorders of glucose metabolism, which resulted in increased insulin sensitivity in hepatocytes, and achieved by enhancing the glucose consumption as well as glycogen synthesis in insulin resistance (IR) hepatpcytes. Subsequently, the expression of key proteins involved in IR was detected by western blotting analysis. Protein tyrosine phosphatase-1B (PTP1B), a negative regulator of insulin signaling, could reduce cellular sensitivity to insulin by inhibiting the phosphorylation of insulin receptor substrate-2 (IRS-2). Results of this study showed that C3G inhibited the increase in PTP1B after high glucose and palmitic acid treatment. And this inhibition was accompanied by increased phosphorylation of IRS proteins. 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引用次数: 0
摘要
背景花青素是一类广泛存在于植物中的天然产物。方法用 0.2 mM PA 和 30 mM 葡萄糖培养 HepG2 和 L02 细胞 24 小时以诱导 IR,用 5 mM 葡萄糖处理细胞作为对照。C57BL/6 J雄性小鼠和db/db雄性小鼠以饲料喂养,并灌胃纯水或氰苷-3-O-葡萄糖苷(C3G)溶液(150 mg/kg/天),连续6周。结果这项研究发现,从红杨梅中提取的花青素 C3G 能缓解葡萄糖代谢紊乱,从而提高肝细胞对胰岛素的敏感性,并通过增强胰岛素抵抗(IR)肝细胞的葡萄糖消耗和糖原合成来实现。随后,通过西部印迹分析检测了参与 IR 的关键蛋白的表达。蛋白酪氨酸磷酸酶-1B(PTP1B)是胰岛素信号转导的负调控因子,可通过抑制胰岛素受体底物-2(IRS-2)的磷酸化降低细胞对胰岛素的敏感性。研究结果表明,C3G 可抑制高糖和棕榈酸处理后 PTP1B 的增加。这种抑制作用伴随着 IRS 蛋白磷酸化的增加。结论这些研究结果表明,C3G 可以缓解体外和体内的 IR,从而提高胰岛素敏感性,这为利用天然膳食生物活性成分调节 T2DM 期间的糖代谢提供了新的思路。
Anti-diabetic effect of anthocyanin cyanidin-3-O-glucoside: data from insulin resistant hepatocyte and diabetic mouse
Background
Anthocyanins are a group of natural products widely found in plants. They have been found to alleviate the disorders of glucose metabolism in type 2 diabetes mellitus (T2DM), while the underlying mechanisms remain unclear.
Methods
HepG2 and L02 cells were incubated with 0.2 mM PA and 30 mM glucose for 24 h to induce IR, and cells treated with 5 mM glucose were used as the control. C57BL/6 J male mice and db/db male mice were fed with a chow diet and gavaged with pure water or cyanidin-3-O-glucoside (C3G) solution (150 mg/kg/day) for 6 weeks.
Results
In this study, the anthocyanin C3G, extracted from red bayberry, was found to alleviate disorders of glucose metabolism, which resulted in increased insulin sensitivity in hepatocytes, and achieved by enhancing the glucose consumption as well as glycogen synthesis in insulin resistance (IR) hepatpcytes. Subsequently, the expression of key proteins involved in IR was detected by western blotting analysis. Protein tyrosine phosphatase-1B (PTP1B), a negative regulator of insulin signaling, could reduce cellular sensitivity to insulin by inhibiting the phosphorylation of insulin receptor substrate-2 (IRS-2). Results of this study showed that C3G inhibited the increase in PTP1B after high glucose and palmitic acid treatment. And this inhibition was accompanied by increased phosphorylation of IRS proteins. Furthermore, the effect of C3G on improving IR in vivo was validated by using a diabetic db/db mouse model.
Conclusion
These findings demonstrated that C3G could alleviate IR in vitro and in vivo to increase insulin sensitivity, which may offer a new insight for regulating glucose metabolism during T2DM by using the natural dietary bioactive components.
期刊介绍:
Nutrition & Diabetes is a peer-reviewed, online, open access journal bringing to the fore outstanding research in the areas of nutrition and chronic disease, including diabetes, from the molecular to the population level.