{"title":"综合全局分析揭示了圈养非洲企鹅肠道微生物群与产卵量之间的相关性","authors":"Jingle Jiang, Di Hu, Enle Pei","doi":"10.1016/j.anireprosci.2024.107448","DOIUrl":null,"url":null,"abstract":"<div><p>The egg production of captive African penguins differs considerably between individuals. An understanding of the physiological differences in African penguins with relatively greater and lesser egg production is meaningful for the captive breeding program of this endangered species. The objective of this study was to investigate differential microbial composition and metabolites in captive African penguins with different egg production. Fecal samples were collected from captive female African penguins during the breeding season. The results of 16 S rRNA gene sequencing showed that African penguins with different egg production had similar microbial diversities, whereas a significant difference was observed between their microbial community structure. African penguins with relatively greater egg production exhibited a higher relative abundance of Alphaproteobacteria, Rhizobiales, Bradyrhizobiaceae, <em>Bradyrhizobium</em> and <em>Bosea</em>. Meanwhile, penguins with relatively lesser egg production had an increased proportion of <em>Klebsiella</em> and <em>Plesiomonas</em>. We further identified a total of 1858 metabolites in female African penguins by liquid chromatography-mass spectrometry analysis. Among these metabolites, 13 kinds of metabolites were found to be significantly differential between African penguins with different egg production. In addition, the correlation analysis revealed that the egg production had significant correlations with most of the differential microbial bacteria and metabolites. Our findings might aid in understanding the potential mechanism underlying the phenomenon of abnormal egg production in captive African penguins, and provide novel insights into the relationship between gut microbiota and reproduction in penguins.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrated omics analysis reveals a correlation between gut microbiota and egg production in captive African penguins (Spheniscus demersus)\",\"authors\":\"Jingle Jiang, Di Hu, Enle Pei\",\"doi\":\"10.1016/j.anireprosci.2024.107448\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The egg production of captive African penguins differs considerably between individuals. An understanding of the physiological differences in African penguins with relatively greater and lesser egg production is meaningful for the captive breeding program of this endangered species. The objective of this study was to investigate differential microbial composition and metabolites in captive African penguins with different egg production. Fecal samples were collected from captive female African penguins during the breeding season. The results of 16 S rRNA gene sequencing showed that African penguins with different egg production had similar microbial diversities, whereas a significant difference was observed between their microbial community structure. African penguins with relatively greater egg production exhibited a higher relative abundance of Alphaproteobacteria, Rhizobiales, Bradyrhizobiaceae, <em>Bradyrhizobium</em> and <em>Bosea</em>. Meanwhile, penguins with relatively lesser egg production had an increased proportion of <em>Klebsiella</em> and <em>Plesiomonas</em>. We further identified a total of 1858 metabolites in female African penguins by liquid chromatography-mass spectrometry analysis. Among these metabolites, 13 kinds of metabolites were found to be significantly differential between African penguins with different egg production. In addition, the correlation analysis revealed that the egg production had significant correlations with most of the differential microbial bacteria and metabolites. Our findings might aid in understanding the potential mechanism underlying the phenomenon of abnormal egg production in captive African penguins, and provide novel insights into the relationship between gut microbiota and reproduction in penguins.</p></div>\",\"PeriodicalId\":7880,\"journal\":{\"name\":\"Animal Reproduction Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-02-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Reproduction Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0378432024000393\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378432024000393","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
人工饲养的非洲企鹅的产卵量在个体之间存在很大差异。了解产蛋量相对较高和较低的非洲企鹅的生理差异,对这一濒危物种的人工繁殖计划很有意义。本研究的目的是调查不同产蛋量的圈养非洲企鹅体内不同的微生物组成和代谢物。研究人员在繁殖季节收集了圈养雌性非洲企鹅的粪便样本。16 S rRNA基因测序结果表明,产卵量不同的非洲企鹅具有相似的微生物多样性,但其微生物群落结构存在显著差异。产蛋量相对较高的非洲企鹅表现出较高的Alphaproteobacteria、Rhizobiales、Bradyrhizobiaceae、Bradyrhizobium和Bosea的相对丰度。与此同时,产卵量相对较少的企鹅体内克雷伯氏菌和普莱西单胞菌的比例有所增加。通过液相色谱-质谱分析,我们进一步鉴定了雌性非洲企鹅体内的1858种代谢物。在这些代谢物中,有13种代谢物在不同产蛋量的非洲企鹅之间存在显著差异。此外,相关分析表明,产蛋量与大多数不同的微生物细菌和代谢物有明显的相关性。我们的研究结果可能有助于理解圈养非洲企鹅异常产蛋现象的潜在机制,并为企鹅肠道微生物群与繁殖之间的关系提供了新的见解。
Integrated omics analysis reveals a correlation between gut microbiota and egg production in captive African penguins (Spheniscus demersus)
The egg production of captive African penguins differs considerably between individuals. An understanding of the physiological differences in African penguins with relatively greater and lesser egg production is meaningful for the captive breeding program of this endangered species. The objective of this study was to investigate differential microbial composition and metabolites in captive African penguins with different egg production. Fecal samples were collected from captive female African penguins during the breeding season. The results of 16 S rRNA gene sequencing showed that African penguins with different egg production had similar microbial diversities, whereas a significant difference was observed between their microbial community structure. African penguins with relatively greater egg production exhibited a higher relative abundance of Alphaproteobacteria, Rhizobiales, Bradyrhizobiaceae, Bradyrhizobium and Bosea. Meanwhile, penguins with relatively lesser egg production had an increased proportion of Klebsiella and Plesiomonas. We further identified a total of 1858 metabolites in female African penguins by liquid chromatography-mass spectrometry analysis. Among these metabolites, 13 kinds of metabolites were found to be significantly differential between African penguins with different egg production. In addition, the correlation analysis revealed that the egg production had significant correlations with most of the differential microbial bacteria and metabolites. Our findings might aid in understanding the potential mechanism underlying the phenomenon of abnormal egg production in captive African penguins, and provide novel insights into the relationship between gut microbiota and reproduction in penguins.
期刊介绍:
Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction.
The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques.
The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.