Hazem M. Ibrahim, Gina M. Mohammed, Rafik Hamed Sayed, Hisham A. Elshoky, Heba Elsayed Elzorkany, Shaimaa Abdelall Elsaady
{"title":"使用纳米材料佐剂提高鸡沙门氏菌三联疫苗的效力","authors":"Hazem M. Ibrahim, Gina M. Mohammed, Rafik Hamed Sayed, Hisham A. Elshoky, Heba Elsayed Elzorkany, Shaimaa Abdelall Elsaady","doi":"10.1186/s43088-024-00477-x","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>This study aimed to develop a vaccine for controlling salmonellosis, a zoonotic disease affecting both humans and chicken, by employing Fe<sub>2</sub>O<sub>3</sub> ferrous iron oxide (FNPs), silicon dioxide (SiNPs), carboxymethyl chitosan (C.CS NPs), and FNPs-chitosan (FCNPs) nanocomposite as immunological adjuvants. The immune response of vaccinated chicken was assessed through ELISA and challenge tests.</p><h3>Results</h3><p>The hydrodynamic diameters of Fe<sub>2</sub>O<sub>3</sub>, Fe<sub>2</sub>O<sub>3</sub>-CS, C.CS, and SiO<sub>2</sub> NPs were found to be 81.95 ± 14.95, 137.1 ± 20.5, 32.86 ± 14.05, and 15.64 ± 3.6 nm, respectively. The incorporation of nanoparticles into the vaccine formulation significantly enhanced its efficacy by eliciting a robust immune response. According to the study, FNPs, SiNPs, C.CS NPs, and FCNPs can be used as immunological adjuvants to strengthen chicken’s immune systems and help prevent salmonellosis. By gradually raising antibody titers, all five vaccine formulations successfully stimulated an immunological response against <i>Salmonella</i> in vaccinated chicken. The size of the immunological response, however, differed amongst the various vaccination formulations. The SiNPs group had the highest antibody titer, followed by the locally administered vaccine.</p><h3>Conclusions</h3><p>These findings suggest that the use of silicon dioxide SiNPs as a vaccine delivery system could enhance the immune response to <i>Salmonella</i> in chicken. Overall, the study demonstrates that the use of adjuvanted vaccines with nanomaterials, particularly SiNPs, has significantly increased the protection rate from 67 to 93.3% when compared to the locally used vaccine, which had a protection rate of 83%.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00477-x","citationCount":"0","resultStr":"{\"title\":\"Efficacy improvement of tri-serotypes vaccine for Salmonella using nanomaterial-based adjuvant in chicken\",\"authors\":\"Hazem M. Ibrahim, Gina M. Mohammed, Rafik Hamed Sayed, Hisham A. Elshoky, Heba Elsayed Elzorkany, Shaimaa Abdelall Elsaady\",\"doi\":\"10.1186/s43088-024-00477-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>This study aimed to develop a vaccine for controlling salmonellosis, a zoonotic disease affecting both humans and chicken, by employing Fe<sub>2</sub>O<sub>3</sub> ferrous iron oxide (FNPs), silicon dioxide (SiNPs), carboxymethyl chitosan (C.CS NPs), and FNPs-chitosan (FCNPs) nanocomposite as immunological adjuvants. The immune response of vaccinated chicken was assessed through ELISA and challenge tests.</p><h3>Results</h3><p>The hydrodynamic diameters of Fe<sub>2</sub>O<sub>3</sub>, Fe<sub>2</sub>O<sub>3</sub>-CS, C.CS, and SiO<sub>2</sub> NPs were found to be 81.95 ± 14.95, 137.1 ± 20.5, 32.86 ± 14.05, and 15.64 ± 3.6 nm, respectively. The incorporation of nanoparticles into the vaccine formulation significantly enhanced its efficacy by eliciting a robust immune response. According to the study, FNPs, SiNPs, C.CS NPs, and FCNPs can be used as immunological adjuvants to strengthen chicken’s immune systems and help prevent salmonellosis. By gradually raising antibody titers, all five vaccine formulations successfully stimulated an immunological response against <i>Salmonella</i> in vaccinated chicken. The size of the immunological response, however, differed amongst the various vaccination formulations. The SiNPs group had the highest antibody titer, followed by the locally administered vaccine.</p><h3>Conclusions</h3><p>These findings suggest that the use of silicon dioxide SiNPs as a vaccine delivery system could enhance the immune response to <i>Salmonella</i> in chicken. 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Efficacy improvement of tri-serotypes vaccine for Salmonella using nanomaterial-based adjuvant in chicken
Background
This study aimed to develop a vaccine for controlling salmonellosis, a zoonotic disease affecting both humans and chicken, by employing Fe2O3 ferrous iron oxide (FNPs), silicon dioxide (SiNPs), carboxymethyl chitosan (C.CS NPs), and FNPs-chitosan (FCNPs) nanocomposite as immunological adjuvants. The immune response of vaccinated chicken was assessed through ELISA and challenge tests.
Results
The hydrodynamic diameters of Fe2O3, Fe2O3-CS, C.CS, and SiO2 NPs were found to be 81.95 ± 14.95, 137.1 ± 20.5, 32.86 ± 14.05, and 15.64 ± 3.6 nm, respectively. The incorporation of nanoparticles into the vaccine formulation significantly enhanced its efficacy by eliciting a robust immune response. According to the study, FNPs, SiNPs, C.CS NPs, and FCNPs can be used as immunological adjuvants to strengthen chicken’s immune systems and help prevent salmonellosis. By gradually raising antibody titers, all five vaccine formulations successfully stimulated an immunological response against Salmonella in vaccinated chicken. The size of the immunological response, however, differed amongst the various vaccination formulations. The SiNPs group had the highest antibody titer, followed by the locally administered vaccine.
Conclusions
These findings suggest that the use of silicon dioxide SiNPs as a vaccine delivery system could enhance the immune response to Salmonella in chicken. Overall, the study demonstrates that the use of adjuvanted vaccines with nanomaterials, particularly SiNPs, has significantly increased the protection rate from 67 to 93.3% when compared to the locally used vaccine, which had a protection rate of 83%.
期刊介绍:
Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.