印度北部三级医院中与急性下呼吸道感染相关的人类博卡病毒的临床过程和分子特征。

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2024-07-23 Epub Date: 2024-02-29 DOI:10.7883/yoken.JJID.2023.251
Subhabrata Sarkar, Mannat Kang, Suresh Kumar Angurana, Shankar Prasad, Ishani Bora, Pankaj Singh, Vikrant Sharma, Meenakshi Rana, Bhartendu Singh, Muralidharan Jayashree, Radha Kanta Ratho
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引用次数: 0

摘要

对 139 名住院儿童的呼吸道样本进行了人类博卡病毒(HBoV)基因组筛查。对阳性样本进行了部分 VP1/VP2 基因测序,然后进行了分子和系统进化分析。7.2%的患者(10/139)HBoV呈阳性。所有 HBoV 阳性患儿均表现为发热,随后出现咳嗽和呼吸困难(90%;9/10)。三名患儿出现多系统病毒性疾病,其中一人死亡。8 名患儿需要重症监护,5 名患儿需要机械通气。HBoV研究菌株部分VP1/VP2基因的核苷酸相同率为97.52%至99.67%。与 HBoVSt1 株系相比,VP1 蛋白中的非同义氨基酸突变为 T591S(n=8)和 Y517S(n=1);与 HBoVSt2 株系相比,VP1 蛋白中的非同义氨基酸突变为 N475S(n=8)和 S591T(n=2)。一株研究菌株出现了 A556P、H556P、I561S 和 M562R 非同义突变。所有研究菌株均属于 HBoV1 型。7 株 HBoV 属于同一系,3 株属于另一系。在进化动力学方面,采用无相关松弛对数正态时钟和贝叶斯天际线树先验的 GTR+I 替代模型显示,核苷酸替代率为 9.0 x 10-4 [95% HPD 区间:3.1 x 10-6, 2.1 x 10-3]/位点/年。临床怀疑和病毒学筛查是识别儿童 HBoV 的必要条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clinical Course and Molecular Characterization of Human Bocavirus Associated with Acute Lower Respiratory Tract Infections in a Tertiary Care Hospital in Northern India.

Respiratory samples from 139 hospitalized children were screened for the human bocavirus (HBoV) genome. Positive samples were sequenced for the partial VP1/VP2 gene followed by molecular and phylogenetic analyses. HBoV positivity was noted in 7.2% (10/139) of patients. All HBoV-positive children presented with fever, cough, and respiratory distress (90%, 9/10). Three children developed multisystemic viral illness, with one fatality. Eight children required intensive care management and five required mechanical ventilation. The nucleotide percent identity of the partial VP1/VP2 gene in the HBoV study strains ranged from 97.52% to 99.67%. Non-synonymous mutations in the VP1 protein were T591S (n = 8) and Y517S (n = 1) in the HBoV St1 strain and N475S (n = 8) and S591T (n = 2) in the HBoV St2 strain. One strain showed A556P, H556P, I561S, and M562R non-synonymous mutations. All the study strains belonged to the HBoV1 type. Seven HBoV strains belonged to the same lineage, and three belonged to another lineage. For evolutionary dynamics, GTR+I substitution model with uncorrelated relaxed lognormal clock and Bayesian Skyline tree prior showed 9.0 × 10-4 (95% highest probability density interval: 3.1 × 10-6, 2.1 × 10-3) nucleotide substitutions per site per year. Clinical suspicion and virological screening are necessary to identify HBoV infections in children.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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