Zhe-Yan Sa, Xiao-Hua Pan, Xiao-Xiang Zhu, Cai-Lian Lan, Long Wan, Lai Luo, Jin-Sen Xu
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Potassium ion-related genes in myocardial tissue were detected by RNA sequencing. The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the control group, cardiomyocyte cross-sectional area in the model group was significantly increased (<i>P</i><0.01), the ST segment was significantly elevated (<i>P</i><0.01), and QT, QTc, QTd and QTcd were all significantly increased (<i>P</i><0.05, <i>P</i><0.01). After EA treatment, cardiomyocyte cross-sectional area was significantly decreased (<i>P</i><0.01), the ST segment was significantly reduced (<i>P</i><0.01), and the QT, QTc, QTcd and QTd were significantly decreased (<i>P</i><0.01, <i>P</i><0.05). RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were identified. Among them, Kchip2 expression was up-regulated most notablely in the EA group. Compared with the control group, the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were significantly decreased (<i>P</i><0.01, <i>P</i><0.05), while those were increased in the EA group (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>EA may improve myocardial electrical remodeling in rats with myocardial infarction, which may be related to its functions in up-regulating the expressions of Kchip2 and Kv4.2.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 2","pages":"155-163"},"PeriodicalIF":0.0000,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of electroacupuncture on myocardial electrical remodeling in rats with acute myocardial infarction.\",\"authors\":\"Zhe-Yan Sa, Xiao-Hua Pan, Xiao-Xiang Zhu, Cai-Lian Lan, Long Wan, Lai Luo, Jin-Sen Xu\",\"doi\":\"10.13702/j.1000-0607.20220908\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To investigate the mechanism of electroacupuncture (EA) at \\\"Neiguan\\\" (PC6) in impro-ving myocardial electrical remodeling in rats with acute myocardial infarction (AMI) by enhancing transient outward potassium current.</p><p><strong>Methods: </strong>A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group. The AMI model was established by subcutaneous injection with isoprenaline (ISO, 85 mg/kg). EA was applied to left PC6 for 20 min, once daily for 5 days. Electrocardiogram (ECG) was recorded after treatment. TTC staining was used to observe myocardial necrosis. HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium. Potassium ion-related genes in myocardial tissue were detected by RNA sequencing. The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the control group, cardiomyocyte cross-sectional area in the model group was significantly increased (<i>P</i><0.01), the ST segment was significantly elevated (<i>P</i><0.01), and QT, QTc, QTd and QTcd were all significantly increased (<i>P</i><0.05, <i>P</i><0.01). After EA treatment, cardiomyocyte cross-sectional area was significantly decreased (<i>P</i><0.01), the ST segment was significantly reduced (<i>P</i><0.01), and the QT, QTc, QTcd and QTd were significantly decreased (<i>P</i><0.01, <i>P</i><0.05). RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were identified. Among them, Kchip2 expression was up-regulated most notablely in the EA group. 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引用次数: 0
摘要
研究目的研究电针 "内关"（PC6）通过增强瞬时外向钾电流改善急性心肌梗死（AMI）大鼠心肌电重塑的机制：将 30 只雄性 SD 大鼠随机分为对照组、模型组和 EA 组，每组 10 只。通过皮下注射异丙肾上腺素（ISO，85 mg/kg）建立 AMI 模型。EA作用于左侧PC6，持续20分钟，每天一次，连续5天。治疗后记录心电图（ECG）。TTC染色用于观察心肌坏死。HE 染色用于观察心肌组织的病理形态并测量心肌的横截面积。通过 RNA 测序检测心肌组织中的钾离子相关基因。实时荧光定量 PCR 和 Western 印迹分别检测心肌组织中 Kchip2 和 Kv4.2 的 mRNA 和蛋白表达：结果：与对照组相比，模型组心肌细胞横截面积明显增加（PPPPPPPPPPPPC结论：EA可改善心肌的心肌功能：EA可改善心肌梗死大鼠的心肌电重构，这可能与其上调Kchip2和Kv4.2的表达有关。
Effect of electroacupuncture on myocardial electrical remodeling in rats with acute myocardial infarction.
Objectives: To investigate the mechanism of electroacupuncture (EA) at "Neiguan" (PC6) in impro-ving myocardial electrical remodeling in rats with acute myocardial infarction (AMI) by enhancing transient outward potassium current.
Methods: A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group. The AMI model was established by subcutaneous injection with isoprenaline (ISO, 85 mg/kg). EA was applied to left PC6 for 20 min, once daily for 5 days. Electrocardiogram (ECG) was recorded after treatment. TTC staining was used to observe myocardial necrosis. HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium. Potassium ion-related genes in myocardial tissue were detected by RNA sequencing. The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.
Results: Compared with the control group, cardiomyocyte cross-sectional area in the model group was significantly increased (P<0.01), the ST segment was significantly elevated (P<0.01), and QT, QTc, QTd and QTcd were all significantly increased (P<0.05, P<0.01). After EA treatment, cardiomyocyte cross-sectional area was significantly decreased (P<0.01), the ST segment was significantly reduced (P<0.01), and the QT, QTc, QTcd and QTd were significantly decreased (P<0.01, P<0.05). RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were identified. Among them, Kchip2 expression was up-regulated most notablely in the EA group. Compared with the control group, the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were significantly decreased (P<0.01, P<0.05), while those were increased in the EA group (P<0.01, P<0.05).
Conclusions: EA may improve myocardial electrical remodeling in rats with myocardial infarction, which may be related to its functions in up-regulating the expressions of Kchip2 and Kv4.2.
期刊介绍：
Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture.
The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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