{"title":"电针督脉对脊髓损伤大鼠线粒体融合和神经干细胞增殖分化的影响","authors":"Ming-Li Wu, Zhao-Yuan Duan, Wen-Tao Chang, Jing Gao, Kai-Qi Su, Xiao-Dong Feng","doi":"10.13702/j.1000-0607.20230375","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) at \"Dazhui\" (GV14) and \"Jizhong\"(GV6) of the Governor Vessel (GV) on mitochondrial fusion and neural stem cell (NSC) proliferation and differentiation in the spinal cord of rats with spinal cord injury (SCI), so as to investigate its mechanisms underlying improvement of SCI.</p><p><strong>Methods: </strong>SD rats were randomly divided into sham operation, model and EA groups, with 15 rats in each group. The SCI model was established by using a precision impactor. EA (20 Hz/100 Hz, 1-2 mA) was applied to GV14 and GV6 for 30 min, once daily for 14 days. The rats' hindlimb locomotor function in each group was assessed using the Basso-Beattie-Bresnahan (BBB) locomotor scale. Histopathological changes of the injured spinal cord tissue and the number of neurons were evaluated after H.E. staining and Nissl staining. The expressions of Nestin, mitochondrial fusion-related protein optic atrophy-1 (OPA1) and NSC markers sex-determining region Y-box 2 (SOX2) in the injured spinal cord tissue were detected by immunofluorescence staining. The protein and mRNA expression levels of Nestin in the spinal cord tissue were detected by quantitative real-time PCR and Western blot, separately.</p><p><strong>Results: </strong>Compared with the sham operation group, the BBB scores after modeling, and the number of neurons were significantly decreased (<i>P<</i>0.001), while the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein considerably increased (<i>P<</i>0.001, <i>P<</i>0.01, <i>P<</i>0.05) in the model group. After EA intervention and in comparison with the model group, the BBB scores at the 7<sup>th</sup> and 14<sup>th</sup> day, the number of neurons, the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein were strikingly increased (<i>P<</i>0.05, <i>P<</i>0.01, <i>P<</i>0.001) in the EA group. H.E. staining showed swollen, ruptured and necrotic neurons of the spinal cord, with a large number of vacuoles and severe inflammatory cell infiltration after modeling, which was relatively milder in the EA group.</p><p><strong>Conclusions: </strong>EA stimulation of GV14 and GV6 can promote the recovery of motor function in rats with SCI, which may be related to its effects in promoting mitochondrial fusion and enhancing the proliferation and differentiation of NSCs.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 2","pages":"119-126"},"PeriodicalIF":0.0000,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of electroacupuncture of Governor Vessel on mitochondrial fusion and proliferation and differentiation of neural stem cells in spinal cord injury rats.\",\"authors\":\"Ming-Li Wu, Zhao-Yuan Duan, Wen-Tao Chang, Jing Gao, Kai-Qi Su, Xiao-Dong Feng\",\"doi\":\"10.13702/j.1000-0607.20230375\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) at \\\"Dazhui\\\" (GV14) and \\\"Jizhong\\\"(GV6) of the Governor Vessel (GV) on mitochondrial fusion and neural stem cell (NSC) proliferation and differentiation in the spinal cord of rats with spinal cord injury (SCI), so as to investigate its mechanisms underlying improvement of SCI.</p><p><strong>Methods: </strong>SD rats were randomly divided into sham operation, model and EA groups, with 15 rats in each group. The SCI model was established by using a precision impactor. EA (20 Hz/100 Hz, 1-2 mA) was applied to GV14 and GV6 for 30 min, once daily for 14 days. The rats' hindlimb locomotor function in each group was assessed using the Basso-Beattie-Bresnahan (BBB) locomotor scale. Histopathological changes of the injured spinal cord tissue and the number of neurons were evaluated after H.E. staining and Nissl staining. The expressions of Nestin, mitochondrial fusion-related protein optic atrophy-1 (OPA1) and NSC markers sex-determining region Y-box 2 (SOX2) in the injured spinal cord tissue were detected by immunofluorescence staining. The protein and mRNA expression levels of Nestin in the spinal cord tissue were detected by quantitative real-time PCR and Western blot, separately.</p><p><strong>Results: </strong>Compared with the sham operation group, the BBB scores after modeling, and the number of neurons were significantly decreased (<i>P<</i>0.001), while the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein considerably increased (<i>P<</i>0.001, <i>P<</i>0.01, <i>P<</i>0.05) in the model group. After EA intervention and in comparison with the model group, the BBB scores at the 7<sup>th</sup> and 14<sup>th</sup> day, the number of neurons, the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein were strikingly increased (<i>P<</i>0.05, <i>P<</i>0.01, <i>P<</i>0.001) in the EA group. H.E. staining showed swollen, ruptured and necrotic neurons of the spinal cord, with a large number of vacuoles and severe inflammatory cell infiltration after modeling, which was relatively milder in the EA group.</p><p><strong>Conclusions: </strong>EA stimulation of GV14 and GV6 can promote the recovery of motor function in rats with SCI, which may be related to its effects in promoting mitochondrial fusion and enhancing the proliferation and differentiation of NSCs.</p>\",\"PeriodicalId\":34919,\"journal\":{\"name\":\"针刺研究\",\"volume\":\"49 2\",\"pages\":\"119-126\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-02-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"针刺研究\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.13702/j.1000-0607.20230375\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"针刺研究","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13702/j.1000-0607.20230375","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Effect of electroacupuncture of Governor Vessel on mitochondrial fusion and proliferation and differentiation of neural stem cells in spinal cord injury rats.
Objectives: To observe the effect of electroacupuncture (EA) at "Dazhui" (GV14) and "Jizhong"(GV6) of the Governor Vessel (GV) on mitochondrial fusion and neural stem cell (NSC) proliferation and differentiation in the spinal cord of rats with spinal cord injury (SCI), so as to investigate its mechanisms underlying improvement of SCI.
Methods: SD rats were randomly divided into sham operation, model and EA groups, with 15 rats in each group. The SCI model was established by using a precision impactor. EA (20 Hz/100 Hz, 1-2 mA) was applied to GV14 and GV6 for 30 min, once daily for 14 days. The rats' hindlimb locomotor function in each group was assessed using the Basso-Beattie-Bresnahan (BBB) locomotor scale. Histopathological changes of the injured spinal cord tissue and the number of neurons were evaluated after H.E. staining and Nissl staining. The expressions of Nestin, mitochondrial fusion-related protein optic atrophy-1 (OPA1) and NSC markers sex-determining region Y-box 2 (SOX2) in the injured spinal cord tissue were detected by immunofluorescence staining. The protein and mRNA expression levels of Nestin in the spinal cord tissue were detected by quantitative real-time PCR and Western blot, separately.
Results: Compared with the sham operation group, the BBB scores after modeling, and the number of neurons were significantly decreased (P<0.001), while the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein considerably increased (P<0.001, P<0.01, P<0.05) in the model group. After EA intervention and in comparison with the model group, the BBB scores at the 7th and 14th day, the number of neurons, the mean fluorescence intensity values of Nestin, SOX2 and OPA1, and the expressions of Nestin mRNA and protein were strikingly increased (P<0.05, P<0.01, P<0.001) in the EA group. H.E. staining showed swollen, ruptured and necrotic neurons of the spinal cord, with a large number of vacuoles and severe inflammatory cell infiltration after modeling, which was relatively milder in the EA group.
Conclusions: EA stimulation of GV14 and GV6 can promote the recovery of motor function in rats with SCI, which may be related to its effects in promoting mitochondrial fusion and enhancing the proliferation and differentiation of NSCs.
期刊介绍：
Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture.
The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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