以机械方式整合坚固的水凝胶-组织混合体以促进生物人工心脏瓣膜抗钙化和内皮化的策略。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-01-30 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae003
Haoshuang Wu, Nuoya Chen, Tiantian Zheng, Li Li, Mengyue Hu, Yumei Qin, Gaoyang Guo, Li Yang, Yunbing Wang
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引用次数: 0

摘要

几十年来,生物人工心脏瓣膜(BHV)置换术一直是治疗严重心脏瓣膜疾病的主要方法。大多数临床可用的生物人工心脏瓣膜都是由戊二醛(GLUT)交联而成,而残留 GLUT 的高毒性会导致钙化、严重血栓形成和内皮化延迟。在这里,我们构建了一种机械整合的坚固水凝胶-组织混合体,以改善 BHV 的性能。其中,重组人源化 III 型胶原蛋白(rhCOLIII)具有抗凝血和促进内皮化的生物活性,首先通过氢键相互作用融入聚乙烯醇(PVA)水凝胶。然后引入单宁酸,以增强 PVA 基水凝胶的机械性能以及水凝胶层与生物衍生组织之间的界面粘合力,因为单宁酸对多种基质都有很强的亲和力。体外和体内实验结果证实,经嵌入 rhCOLIII 和 TA 的强力 PVA 基水凝胶修饰的 GLUT 交联 BHV 具有长期抗凝、加速内皮化、轻度炎症反应和抗钙化的特性。因此,我们的机械整合鲁棒性水凝胶-组织混合策略显示出了增强 BHVs 植入后的服务功能和延长其使用寿命的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A strategy for mechanically integrating robust hydrogel-tissue hybrid to promote the anti-calcification and endothelialization of bioprosthetic heart valve.

Bioprosthetic heart valve (BHV) replacement has been the predominant treatment for severe heart valve diseases over decades. Most clinically available BHVs are crosslinked by glutaraldehyde (GLUT), while the high toxicity of residual GLUT could initiate calcification, severe thrombosis, and delayed endothelialization. Here, we construed a mechanically integrating robust hydrogel-tissue hybrid to improve the performance of BHVs. In particular, recombinant humanized collagen type III (rhCOLIII), which was precisely customized with anti-coagulant and pro-endothelialization bioactivity, was first incorporated into the polyvinyl alcohol (PVA)-based hydrogel via hydrogen bond interactions. Then, tannic acid was introduced to enhance the mechanical performance of PVA-based hydrogel and interfacial bonding between the hydrogel layer and bio-derived tissue due to the strong affinity for a wide range of substrates. In vitro and in vivo experimental results confirmed that the GLUT-crosslinked BHVs modified by the robust PVA-based hydrogel embedded rhCOLIII and TA possessed long-term anti-coagulant, accelerated endothelialization, mild inflammatory response and anti-calcification properties. Therefore, our mechanically integrating robust hydrogel-tissue hybrid strategy showed the potential to enhance the service function and prolong the service life of the BHVs after implantation.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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