使用 HiBiT 标记病毒样颗粒的麻疹病毒中和抗体快速定量检测系统。

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2024-02-27 DOI:10.1111/1348-0421.13122

Takashi Okura, Kei Miyakawa, Maino Tahara, Kenji Someya, Fumio Seki, Mayuko Nishi, Noriyuki Otsuki, Akihide Ryo

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引用次数: 0

摘要

检测中和抗体（NAbs）的免疫学测试在麻疹（MV）感染控制中非常重要。目前，斑块还原中和试验是测量实际病毒 NAbs 的唯一可靠方法；然而，其可行性受到周转时间长、通量低以及需要实验室生物安全设备等缺点的阻碍。为了解决这些问题，我们开发了一种简单、快速的中病毒 NAb 检测系统，该系统使用基于慢病毒的病毒样颗粒，其外表面含有由中病毒融合蛋白和血凝素组成的 NanoLuc 片段肽 HiBiT。总之，这种简单、安全、快速的方法可用于检测中病毒 NAbs。

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Rapid quantitative detection system for measles virus-neutralizing antibodies using HiBiT-tagged virus-like particles

Immunological testing to detect neutralizing antibodies (NAbs) is important in measles (MV) infection control. Currently, the plaque reduction neutralization test is the only credible method for measuring actual virus NAbs; however, its feasibility is hampered by drawbacks, such as long turnaround times, low throughput, and the need for laboratory biosafety equipment. To solve these problems, we developed a simple and rapid MV-NAb detection system using lentivirus-based virus-like particles incorporated with the NanoLuc fragment peptide HiBiT comprising the MV fusion protein and hemagglutinin on their exterior surface. Overall, this simple, safe, and rapid method could be used to detect MV NAbs.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.