{"title":"传统富含白细胞和血小板的纤维蛋白与低速高级富含血小板的纤维蛋白的血管生成功效比较：体外绒毛膜试验研究。","authors":"Malavika Ravindranath Nair, Raison Thomas, Rucha Shah, Indresh Gowda, Triveni Mavinakote Gowda","doi":"10.17219/dmp/150655","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Platelet-rich fibrin (PRF) is widely used in periodontics for its wound healing potential. Two major variations of PRF are the original leukocyteand platelet-rich fibrin (L-PRF) and the modified lowspeed advanced PRF (A-PRF).</p><p><strong>Objectives: </strong>The aim of the present study was to evaluate and compare the conventional L-PRF protocol and the low-speed A-PRF protocol in terms of angiogenic potential of PRF, using an in vivo chorioallantoic membrane (CAM) assay.</p><p><strong>Material and methods: </strong>Fifteen fertile Giriraja eggs were procured and after a 3-day incubation period, randomly allotted into 3 groups: control; L-PRF; and A-PRF. A total of 20 mL of blood was collected from systemically healthy male volunteers aged 18-24 years, using a standard protocol. The PRF samples were inoculated on the CAM of the eggs. On the 10th day, the eggs were reopened and photographed. The parameters assessed were the number, length, size, and density of blood vessels, as well as the number of junctions formed. The photographs were analyzed using the ImageJ and ProgRes® CapturePro software.</p><p><strong>Results: </strong>Seven days after inoculation, both the A-PRF and L-PRF groups exhibited significantly better results than the control group in terms of the number (59.20 ±6.61 vs. 48.80 ±5.07 vs. 19.20 ±6.98), length (25,000 ±1,813.10 μm vs. 17,000 ±282.90 μm vs. 8,000 ±184.49 μm), size (230,000 ±15,054.00 μm2 vs. 200,000 ±8,295.27 μm2 vs. 150,000 ±4,105.16 μm2), and density (central: 9,100 ±296.78 vs. 5,370 ±272.42 vs. 1,420 ±564.36; peripheral: 9,094 ±400.14 vs. 3,370 ±479.39 vs. 5,420 ±746.73) of blood vessels, as well as the number of junctions formed (52 ±3.81 vs. 41 ±1.58 vs. 33 ±4.64), respectively.</p><p><strong>Conclusions: </strong>The angiogenic potential was increased by the exposure to both L-PRF and A-PRF. However, A-PRF demonstrated statistically significant benefits in terms of the number, length, size, and density of blood vessels, as well as the number of junctions formed in comparison with the control and L-PRF groups.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of the angiogenic efficacy of conventional leukocyte- and platelet-rich fibrin versus low-speed advanced platelet-rich fibrin: An in vitro chorioallantoic membrane assay study.\",\"authors\":\"Malavika Ravindranath Nair, Raison Thomas, Rucha Shah, Indresh Gowda, Triveni Mavinakote Gowda\",\"doi\":\"10.17219/dmp/150655\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Platelet-rich fibrin (PRF) is widely used in periodontics for its wound healing potential. Two major variations of PRF are the original leukocyteand platelet-rich fibrin (L-PRF) and the modified lowspeed advanced PRF (A-PRF).</p><p><strong>Objectives: </strong>The aim of the present study was to evaluate and compare the conventional L-PRF protocol and the low-speed A-PRF protocol in terms of angiogenic potential of PRF, using an in vivo chorioallantoic membrane (CAM) assay.</p><p><strong>Material and methods: </strong>Fifteen fertile Giriraja eggs were procured and after a 3-day incubation period, randomly allotted into 3 groups: control; L-PRF; and A-PRF. A total of 20 mL of blood was collected from systemically healthy male volunteers aged 18-24 years, using a standard protocol. The PRF samples were inoculated on the CAM of the eggs. On the 10th day, the eggs were reopened and photographed. The parameters assessed were the number, length, size, and density of blood vessels, as well as the number of junctions formed. The photographs were analyzed using the ImageJ and ProgRes® CapturePro software.</p><p><strong>Results: </strong>Seven days after inoculation, both the A-PRF and L-PRF groups exhibited significantly better results than the control group in terms of the number (59.20 ±6.61 vs. 48.80 ±5.07 vs. 19.20 ±6.98), length (25,000 ±1,813.10 μm vs. 17,000 ±282.90 μm vs. 8,000 ±184.49 μm), size (230,000 ±15,054.00 μm2 vs. 200,000 ±8,295.27 μm2 vs. 150,000 ±4,105.16 μm2), and density (central: 9,100 ±296.78 vs. 5,370 ±272.42 vs. 1,420 ±564.36; peripheral: 9,094 ±400.14 vs. 3,370 ±479.39 vs. 5,420 ±746.73) of blood vessels, as well as the number of junctions formed (52 ±3.81 vs. 41 ±1.58 vs. 33 ±4.64), respectively.</p><p><strong>Conclusions: </strong>The angiogenic potential was increased by the exposure to both L-PRF and A-PRF. However, A-PRF demonstrated statistically significant benefits in terms of the number, length, size, and density of blood vessels, as well as the number of junctions formed in comparison with the control and L-PRF groups.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17219/dmp/150655\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17219/dmp/150655","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
背景：富血小板纤维蛋白（PRF）因其伤口愈合潜力而广泛应用于牙周病学。富血小板纤维蛋白的两种主要变体是原始的白细胞和富血小板纤维蛋白（L-PRF）和改良的低速高级 PRF（A-PRF）：本研究的目的是利用体内绒毛膜（CAM）试验，评估和比较传统 L-PRF 方案和低速 A-PRF 方案 PRF 的血管生成潜力：采集 15 枚可育 Giriraja 卵，经过 3 天孵化期后随机分为 3 组：对照组、L-PRF 组和 A-PRF 组。按照标准方案，从 18-24 岁全身健康的男性志愿者身上共采集 20 毫升血液。将 PRF 样品接种到卵的 CAM 上。第 10 天，重新打开虫卵并拍照。评估参数包括血管的数量、长度、大小和密度，以及形成的连接点数量。使用 ImageJ 和 ProgRes® CapturePro 软件对照片进行分析：接种七天后，A-PRF 组和 L-PRF 组在数量（59.20 ±6.61 vs. 48.80 ±5.07 vs. 19.20 ±6.98）、长度（25,000 ±1,813.10μm vs. 17,000 ±282.90 μm vs. 8,000 ±184.49 μm）、大小（230,000 ±15,054.00 μm2 vs. 200,000 ±8,295 μm2200,000 ±8,295.27 μm2 vs. 150,000 ±4,105.16 μm2）和密度（中心：9,100 ±296.78 vs. 5,370 ±272.42 vs. 1,420±564.36；外围：9,094 ±400.14 vs. 3,370 ±479.36；中心：5,370 ±272.42 vs. 1,420±564.36）。结论：L-PRF和A-PRF都能增加血管生成潜能。然而，与对照组和 L-PRF 组相比，A-PRF 在血管数量、长度、大小和密度以及形成的连接点数量方面都有显著的统计学优势。
Comparison of the angiogenic efficacy of conventional leukocyte- and platelet-rich fibrin versus low-speed advanced platelet-rich fibrin: An in vitro chorioallantoic membrane assay study.
Background: Platelet-rich fibrin (PRF) is widely used in periodontics for its wound healing potential. Two major variations of PRF are the original leukocyteand platelet-rich fibrin (L-PRF) and the modified lowspeed advanced PRF (A-PRF).
Objectives: The aim of the present study was to evaluate and compare the conventional L-PRF protocol and the low-speed A-PRF protocol in terms of angiogenic potential of PRF, using an in vivo chorioallantoic membrane (CAM) assay.
Material and methods: Fifteen fertile Giriraja eggs were procured and after a 3-day incubation period, randomly allotted into 3 groups: control; L-PRF; and A-PRF. A total of 20 mL of blood was collected from systemically healthy male volunteers aged 18-24 years, using a standard protocol. The PRF samples were inoculated on the CAM of the eggs. On the 10th day, the eggs were reopened and photographed. The parameters assessed were the number, length, size, and density of blood vessels, as well as the number of junctions formed. The photographs were analyzed using the ImageJ and ProgRes® CapturePro software.
Results: Seven days after inoculation, both the A-PRF and L-PRF groups exhibited significantly better results than the control group in terms of the number (59.20 ±6.61 vs. 48.80 ±5.07 vs. 19.20 ±6.98), length (25,000 ±1,813.10 μm vs. 17,000 ±282.90 μm vs. 8,000 ±184.49 μm), size (230,000 ±15,054.00 μm2 vs. 200,000 ±8,295.27 μm2 vs. 150,000 ±4,105.16 μm2), and density (central: 9,100 ±296.78 vs. 5,370 ±272.42 vs. 1,420 ±564.36; peripheral: 9,094 ±400.14 vs. 3,370 ±479.39 vs. 5,420 ±746.73) of blood vessels, as well as the number of junctions formed (52 ±3.81 vs. 41 ±1.58 vs. 33 ±4.64), respectively.
Conclusions: The angiogenic potential was increased by the exposure to both L-PRF and A-PRF. However, A-PRF demonstrated statistically significant benefits in terms of the number, length, size, and density of blood vessels, as well as the number of junctions formed in comparison with the control and L-PRF groups.
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