含虾青素的海藻酸壳聚糖凝胶珠可激活 Nrf2 和促细胞凋亡信号通路，对抗氧化应激。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-03-01 Epub Date: 2024-02-27 DOI:10.1080/02652048.2024.2319048

Hamieh Goshtasbi, Elaheh Dalir Abdolahinia, Marziyeh Fathi, Ali Movafeghi, Hossein Omidian, Jaleh Barar, Yadollah Omidi

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引用次数: 0

摘要

氧化应激（OS）在疾病发展中起着至关重要的作用。虾青素（ATX）是一种珍贵的天然化合物，可减少氧化应激，并可用于治疗神经退行性疾病和癌症等疾病。核因子红细胞2相关因子2（Nrf2）调节抗氧化酶和OS管理。我们通过 Alg-CS/ATX 凝胶珠在体外评估了 ATX 的抗氧化活性。我们合成了 ATX 包裹的海藻酸壳聚糖（Alg-CS/ATX）凝胶珠，并通过扫描电镜、傅立叶变换红外光谱和 XRD 对其结构/形态进行了表征。通过 MTT 试验、Annexin V/PI、细胞周期研究和 Western 印迹法检测了它们在经 H2O2 处理的人脐静脉内皮细胞（HUVECs）中的生物效应。Alg-CS 可有效携带 ATX，且容量大、孔径小。Alg-CS/ATX 的封装效率高达 84%，稳定性可维持 30 天。体外研究显示，在 pH 值为 5.4 时，ATX 的释放速度是中性 pH 值时的 1.4 倍，从而提高了 ATX 的治疗潜力。用 Alg-CS/ATX 处理的 HUVEC 通过增加 Nrf2 的表达提高了存活率。作为一种生物相容性载体，Alg-CS凝胶珠在递送ATX以抗击OS方面表现出了巨大的潜力，具有相当大的临床应用前景。

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Astaxanthin-loaded alginate-chitosan gel beads activate Nrf2 and pro-apoptotic signalling pathways against oxidative stress.

Oxidative stress (OS) plays a crucial role in disease development. Astaxanthin (ATX), a valuable natural compound, may reduce OS and serve as a treatment for diseases like neurodegenerative disorders and cancer. Nuclear factor-erythroid 2-related factor 2 (Nrf2) regulates antioxidant enzymes and OS management. We evaluated ATX's antioxidant activity via Alg-CS/ATX gel beads in vitro. ATX-encapsulated alginate-chitosan (Alg-CS/ATX) gel beads were synthesized and structurally/morphologically characterized by SEM, FT-IR, and XRD. Their biological effects were examined in human umbilical vein endothelial cells (HUVECs) treated with H₂O₂ through MTT assay, Annexin V/PI, cell cycle studies, and western blotting. Alg-CS effectively carried ATX, with high capacity and reduced pore size. Alg-CS/ATX displayed an 84% encapsulation efficiency, maintaining stability for 30 days. In vitro studies showed a 1.4-fold faster release at pH 5.4 than at neutral pH, improving ATX's therapeutic potential. HUVECs treated with Alg-CS/ATX showed enhanced viability via increased Nrf2 expression. Alg-CS gel beads exhibit significant potential as a biocompatible vehicle for delivering ATX to combat OS with considerable opportunity for clinical applications.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.