在伊朗西北部小反刍动物的蜱虫和血液样本中进行烧伤柯西氏菌的分子检测。

IF 1.8 2区 农林科学 Q2 ENTOMOLOGY
Experimental and Applied Acarology Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI:10.1007/s10493-023-00888-y
Ahmad Enferadi Ghazanabad, Negin Esfandiari, Mohammadreza Najafi, Shahryar Mehrabi, Saeedeh Sarani, Peyman Khademi, Max Maurin
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引用次数: 0

摘要

这项调查旨在通过分子方法检测西阿塞拜疆(伊朗西北部)地区小反刍动物身上附着的 Argasidae 和 Ixodidae 蜱以及从相同动物身上采集的血液样本中的烧伤柯西氏菌。从绵羊(n = 536)和山羊(n = 391)身上采集了 451 份蜱虫样本和 927 份血液样本,并通过巢式 PCR 检测烧伤蜱插入序列 IS1111 或 icd 基因序列。采集到的蜱虫从形态上分为 Rhipicephalus sanguineus、Rhipicephalus turanicus、Hyalomma asiaticum、Hyalomma anatolicum 或 Argas reflexus。14%的蜱虫(共 65 只,其中 43 只的 IS1111 检测呈阳性,22 只的 icd 基因检测呈阳性)的烧伤蜱检测呈阳性,其中没有一只来自 Argas 属。在 927 份血液样本中,有 218 份(23.5%)对烧伤蜱呈阳性检测结果。针对 IS1111 和 icd 基因的分析结果呈阳性的分别为 131 例和 87 例。由于 Q 热是一种蜱传动物传染病,并且是伊朗的地方病,这些信息对于制定有效、协调和战略性的蜱和病原体控制计划以防止疾病在家畜和人类中爆发至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular detection of Coxiella burnetii in tick and blood samples from small ruminants in northwest of Iran.

This survey sought to molecularly detect Coxiella burnetii in Argasidae and Ixodidae ticks attached to small ruminants in the region of West Azerbaijan (Northwest of Iran) and blood samples collected from the same animals. 451 tick samples and 927 blood samples were obtained from sheep (n = 536) and goats (n = 391) and tested by nested PCR for detection of C. burnetii insertion sequence IS1111 or icd gene sequence. The collected ticks were morphologically classified as Rhipicephalus sanguineus, Rhipicephalus turanicus, Hyalomma asiaticum, Hyalomma anatolicum, or Argas reflexus. 14% of ticks (65 in total 43 for IS1111 and 22 for icd gene) tested positive for C. burnetii, none of which were from the Argas genus. Among the 927 blood samples, 218 (23.5%) tested positive for C. burnetii. The positive result from analysis targeting the genes IS1111 and icd were 131 and 87 respectively. As Q fever is a tickborne zoonosis and endemic to Iran, such information is critical for creating effective, coordinated, and strategic tick and pathogen control programs to prevent disease outbreak in domestic animals and humans.

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来源期刊
CiteScore
3.90
自引率
9.10%
发文量
81
审稿时长
4-8 weeks
期刊介绍: Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.
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