利用环氧树脂光刻胶微加工出独立的、可调节的多孔微过滤器，实现有效的生物分离。

IF 1.6 4区医学 Q4 BIOPHYSICS

Biointerphases Pub Date : 2024-01-01 DOI:10.1116/6.0003165

Sudeshna Sengupta, D Shyamala, Sivasundari Kannan, V T Fidal Kumar, Enakshi Bhattacharya

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引用次数: 0

摘要

SU-8 是一种环氧基生物相容性热固性聚合物，主要用于制造生物医学设备和支架。在这项研究中，对单层独立可调多孔 SU-8 薄膜进行了微加工和表面亲水处理，以实现高效的生物分离。与以往 200-300 μm 的较厚膜不同，这种厚度为 50-60 μm、孔径为 6-10 μm 的 SU-8 薄膜是在没有任何额外支撑结构的情况下制造并测试用于血浆分离的。该方法的基础是在硅晶片上附着的牺牲聚对苯二甲酸乙二醇酯（PET）薄片上，通过电泳涂布和紫外光刻制作图案化的 SU-8 层。PET 和 SU-8 之间的粘附性较差，有助于方便地释放形成均匀孔隙的薄多孔膜。单层自支撑膜坚固、安全、可灭菌、可重复使用，适用于血浆分离和发酵后肉汤富集。

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Microfabricated free standing, tuneable, porous microfilters from an epoxy based photoresist for effective bioseparation.

SU-8 is an epoxy-based, biocompatible thermosetting polymer, which has been utilized mainly to fabricate biomedical devices and scaffolds. In this study, thin, single-layered, freestanding tuneable porous SU-8 membranes were microfabricated and surface hydrophilized for efficient bioseparation. Unlike the previous thicker membranes of 200-300 μm, these thin SU-8 membranes of 50-60 μm thickness and pores with 6-10 μm diameter were fabricated and tested for blood-plasma separation, without any additional support structure. The method is based on making a patterned SU-8 layer by electrospin coating and UV lithography on a sacrificial polyethylene terephthalate (PET) sheet attached to a silicon wafer. Poor adhesion between PET and SU-8 aid in the convenient release of the thin porous membranes with uniform pore formation. The single-layered self-supporting membranes were strong, safe, sterilizable, reusable, and suitable for plasma separation and postfermentation broth enrichment.

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来源期刊

Biointerphases 生物-材料科学：生物材料

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期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.