The impact of magnesium sulfate on the gene expression of Bordetella pertussis (ptxP1 and ptxP3) strains and the incidence of whooping cough in Nineveh

Introduction

The incidence of Bordetella pertussis infections in the Nineveh is affected by living conditions and the density of the population in specific geographic regions.

Aim

This study amid to assess the monitoring pertussis in Nineveh between 2017 and 2021.

Methods

This work focused on how MgSO4 impacts the activity of ptxP1 and ptxP3 genes in B. pertussis strains.

Results

Mosul, Al-Baaj, and Sinjar recorded higher rates of infection compared to other regions due to population density. The expression BfrD, ptxE, ptxD, ptxB, ptxA, and ptxC was exhibited elevation when exposed to MgSO4 of 0 and 5 mM conc. On the other hand, WbpT, VipC, and KpsM showed high expression at a concentration of 50 mM of MgSO4. Pgm demonstrated elevated expression across all concentrations of MgSO4 for the ptxP1 strain, while maintaining consistently low expression for the ptxP3 strain. The potential of Pgm as a distinct marker, effectively distinguishing between the 2 strains of B. pertussis. This approach will allow for a more detailed exploration of the variations between bacterial strains regarding their genetic responses to MgSO4 concentrations.

Conclusions

Data analysis of microarray was indicated that ptxP3 strains are less affected by the inhibitory effects of sulfate root on undesirable gene expression. This investigation may lead for further studies into the role of sulfite root in pertussis's pathogenicity.