{"title":"两种义齿硬衬材料对人牙龈成纤维细胞细胞毒性作用的比较分析：体外研究","authors":"Somayeh Hashemi , Reza Nahidi , Homeyra Ansari , Kiarash Firoozi , Rata Rokhshad","doi":"10.1016/j.sdentj.2024.02.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The objective of this study was to compare the cytotoxicity of TDV and Rebase II denture hard liners on human gingival fibroblasts, aiming to address issues associated with incomplete polymerization and free monomers that affect material properties.</p></div><div><h3>Methods</h3><p>Seventy-two specimens (24 each of TDV, Rebase II, and controls) were prepared under aseptic conditions according to factory instructions. Cytotoxicity was determined using the MTT test with methyl tetrazolium salt added to the cell culture medium. A two-way ANOVA and a post-hoc Tukey test was used to evaluate the results of incubation before mitochondrial activity was measured using Multiscan spectrophotometry (570 nm).</p></div><div><h3>Results</h3><p>There were significant differences in cell viability between the groups after 24 hours (<em>P</em> < 0.001), with TDV having higher viability than Rebase II. The difference between Rebase II and TDV, however, was not significant at 48 and 96 hours (<em>P</em> > 0.131). At 24 hours, Rebase II exhibited significantly lower viability than TDV liner, with a significant difference between the two groups (<em>P</em> = 0.001).</p></div><div><h3>Conclusion</h3><p>Due to the maximum monomer release in the early hours of incubation, the amount of cytotoxicity decreased with increasing incubation time.</p></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 5","pages":"Pages 765-769"},"PeriodicalIF":1.7000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1013905224000579/pdfft?md5=59ac1a440f857db1496a393b600d79b8&pid=1-s2.0-S1013905224000579-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Comparative analysis of cytotoxicity effects of two denture hard lining materials on human gingival fibroblasts: an in vitro study\",\"authors\":\"Somayeh Hashemi , Reza Nahidi , Homeyra Ansari , Kiarash Firoozi , Rata Rokhshad\",\"doi\":\"10.1016/j.sdentj.2024.02.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The objective of this study was to compare the cytotoxicity of TDV and Rebase II denture hard liners on human gingival fibroblasts, aiming to address issues associated with incomplete polymerization and free monomers that affect material properties.</p></div><div><h3>Methods</h3><p>Seventy-two specimens (24 each of TDV, Rebase II, and controls) were prepared under aseptic conditions according to factory instructions. Cytotoxicity was determined using the MTT test with methyl tetrazolium salt added to the cell culture medium. A two-way ANOVA and a post-hoc Tukey test was used to evaluate the results of incubation before mitochondrial activity was measured using Multiscan spectrophotometry (570 nm).</p></div><div><h3>Results</h3><p>There were significant differences in cell viability between the groups after 24 hours (<em>P</em> < 0.001), with TDV having higher viability than Rebase II. The difference between Rebase II and TDV, however, was not significant at 48 and 96 hours (<em>P</em> > 0.131). At 24 hours, Rebase II exhibited significantly lower viability than TDV liner, with a significant difference between the two groups (<em>P</em> = 0.001).</p></div><div><h3>Conclusion</h3><p>Due to the maximum monomer release in the early hours of incubation, the amount of cytotoxicity decreased with increasing incubation time.</p></div>\",\"PeriodicalId\":47246,\"journal\":{\"name\":\"Saudi Dental Journal\",\"volume\":\"36 5\",\"pages\":\"Pages 765-769\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1013905224000579/pdfft?md5=59ac1a440f857db1496a393b600d79b8&pid=1-s2.0-S1013905224000579-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Saudi Dental Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1013905224000579\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Saudi Dental Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1013905224000579","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景本研究的目的是比较 TDV 和 Rebase II 义齿硬衬对人类牙龈成纤维细胞的细胞毒性，旨在解决与不完全聚合和影响材料特性的游离单体有关的问题。细胞毒性采用在细胞培养基中加入甲基四氮唑盐的 MTT 试验进行测定。在使用 Multiscan 分光光度法（570 nm）测量线粒体活性之前，采用双向方差分析和事后 Tukey 检验来评估培养结果。结果24 小时后，各组细胞存活率存在显著差异（P <0.001），TDV 的存活率高于 Rebase II。然而，在 48 小时和 96 小时时，Rebase II 和 TDV 之间的差异并不明显（P > 0.131）。在 24 小时内，Rebase II 的存活率明显低于 TDV 内衬，两组之间差异显著（P = 0.001）。
Comparative analysis of cytotoxicity effects of two denture hard lining materials on human gingival fibroblasts: an in vitro study
Background
The objective of this study was to compare the cytotoxicity of TDV and Rebase II denture hard liners on human gingival fibroblasts, aiming to address issues associated with incomplete polymerization and free monomers that affect material properties.
Methods
Seventy-two specimens (24 each of TDV, Rebase II, and controls) were prepared under aseptic conditions according to factory instructions. Cytotoxicity was determined using the MTT test with methyl tetrazolium salt added to the cell culture medium. A two-way ANOVA and a post-hoc Tukey test was used to evaluate the results of incubation before mitochondrial activity was measured using Multiscan spectrophotometry (570 nm).
Results
There were significant differences in cell viability between the groups after 24 hours (P < 0.001), with TDV having higher viability than Rebase II. The difference between Rebase II and TDV, however, was not significant at 48 and 96 hours (P > 0.131). At 24 hours, Rebase II exhibited significantly lower viability than TDV liner, with a significant difference between the two groups (P = 0.001).
Conclusion
Due to the maximum monomer release in the early hours of incubation, the amount of cytotoxicity decreased with increasing incubation time.
期刊介绍：
Saudi Dental Journal is an English language, peer-reviewed scholarly publication in the area of dentistry. Saudi Dental Journal publishes original research and reviews on, but not limited to: • dental disease • clinical trials • dental equipment • new and experimental techniques • epidemiology and oral health • restorative dentistry • periodontology • endodontology • prosthodontics • paediatric dentistry • orthodontics and dental education Saudi Dental Journal is the official publication of the Saudi Dental Society and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.
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