综合浮游植物群落组成方法,促进全球尺度应用

IF 2.1 3区 地球科学 Q2 LIMNOLOGY
Sasha J. Kramer, Luis M. Bolaños, Dylan Catlett, Alison P. Chase, Michael J. Behrenfeld, Emmanuel S. Boss, E. Taylor Crockford, Stephen J. Giovannoni, Jason R. Graff, Nils Haëntjens, Lee Karp-Boss, Emily E. Peacock, Collin S. Roesler, Heidi M. Sosik, David A. Siegel
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引用次数: 0

摘要

海洋浮游植物群落的组成对许多生物地球化学过程和海洋生态系统服务都有影响。有多种方法可用于描述浮游植物群落组成(PCC),其分类学分辨率各不相同。因此,PCC 的测定结果取决于所使用的方法。在此,我们利用在北大西洋和北太平洋采集的表层海洋样本,将基于高效液相色谱色素的 PCC 与其他四种方法(定量细胞成像、流式细胞仪、16S 和 18S rRNA 扩增子测序)进行了比较。通过这些方法,可以确定原核生物和真核生物在各种大小类别中的 PCC 特征。许多类群(如硅藻)的 PCC 估计值在不同的方法中显示出很强的正相关性,而其他类群(如甲藻)则没有被一种或多种方法很好地捕捉到。由于浮游植物色素浓度的变化与光学特性的变化有关,这一组合数据集通过将属和种级的 PCC 与组或类级的色素 PCC 联系起来,扩大了海洋颜色遥感的潜在范围。量化基于色素的 PCC 方法与扩增子测序、成像和细胞计方法的 PCC 评估相比所具有的优势和局限性,是对从空间量化 PCC 的遥感方法进行有力验证的第一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Toward a synthesis of phytoplankton community composition methods for global-scale application

Toward a synthesis of phytoplankton community composition methods for global-scale application

The composition of the marine phytoplankton community has been shown to impact many biogeochemical processes and marine ecosystem services. A variety of methods exist to characterize phytoplankton community composition (PCC), with varying degrees of taxonomic resolution. Accordingly, the resulting PCC determinations are dependent on the method used. Here, we use surface ocean samples collected in the North Atlantic and North Pacific Oceans to compare high-performance liquid chromatography pigment-based PCC to four other methods: quantitative cell imaging, flow cytometry, and 16S and 18S rRNA amplicon sequencing. These methods allow characterization of both prokaryotic and eukaryotic PCC across a wide range of size classes. PCC estimates of many taxa resolved at the class level (e.g., diatoms) show strong positive correlations across methods, while other groups (e.g., dinoflagellates) are not well captured by one or more methods. Since variations in phytoplankton pigment concentrations are related to changes in optical properties, this combined dataset expands the potential scope of ocean color remote sensing by associating PCC at the genus- and species-level with group- or class-level PCC from pigments. Quantifying the strengths and limitations of pigment-based PCC methods compared to PCC assessments from amplicon sequencing, imaging, and cytometry methods is the first step toward the robust validation of remote sensing approaches to quantify PCC from space.

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来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
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