Rust HUBB:基于 DNA 条形码的 Pucciniales 鉴定。

IF 5.2 1区 生物学 Q1 MYCOLOGY
Patricia Kaishian, Christopher R K Layug, Mark Anderson, Diane R Berg, M Catherine Aime
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引用次数: 0

摘要

锈菌(Pucciniales,Basidiomycota)是一种物种丰富(约 8000 种)、分布于全球的强制性植物病原体。锈菌具有宿主特异性,作为一个群体,它们会对许多最具经济和/或生态意义的植物造成病害。因此,真菌学家、植物学家、农业科学家、农民、检疫官员和相关利益方都对准确、快速地识别这些真菌的能力特别感兴趣。然而,锈病的生命周期非常复杂,可能会产生多达五种不同的孢子类型,并在两种互不相关的寄主物种之间交替出现,这使得标准鉴定,尤其是对记录较少的孢子状态或交替寄主的鉴定极为困难。普渡大学的亚瑟真菌标本馆(PUR)是世界上收藏锈病真菌最全面的标本馆之一。利用 PUR 中的凭证材料和新鲜采集物,我们从超过 3700 份锈病真菌标本中生成了 28S 核糖体重复的 DNA 条形码。条形码材料涵盖 120 个属和超过 1100 个种,其中大多数都有多个重复序列。条形码和相关元数据已存入一个名为 Rust HUBB(基于标本馆的通用条形码 Blast)的可公开访问的 BLAST 搜索数据库中,并将不断更新。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rust HUBB: DNA barcode-based identification of Pucciniales.

Rust fungi (Pucciniales, Basidiomycota) are a species-rich (ca. 8000 species), globally distributed order of obligate plant pathogens. Rust species are host-specific, and as a group they cause disease on many of our most economically and/or ecologically significant plants. As such, the ability to accurately and rapidly identify these fungi is of particular interest to mycologists, botanists, agricultural scientists, farmers, quarantine officials, and associated stakeholders. However, the complexities of the rust life cycle, which may include production of up to five different spore types and alternation between two unrelated host species, have made standard identifications, especially of less-documented spore states or alternate hosts, extremely difficult. The Arthur Fungarium (PUR) at Purdue University is home to one of the most comprehensive collections of rust fungi in the world. Using material vouchered in PUR supplemented with fresh collections we generated DNA barcodes of the 28S ribosomal repeat from > 3700 rust fungal specimens. Barcoded material spans 120 genera and > 1100 species, most represented by several replicate sequences. Barcodes and associated metadata are hosted in a publicly accessible, BLAST searchable database called Rust HUBB (Herbarium-based Universal Barcode Blast) and will be continuously updated.

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来源期刊
Ima Fungus
Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍: The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue
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