乙烯-MPK8-ERF.C1-PR模块赋予番茄果实抗灰霉病的能力，同时不影响成熟。

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-02-25 DOI:10.1111/nph.19632

Heng Deng, Yangang Pei, Xin Xu, Xiaofei Du, Qihan Xue, Zhuo Gao, Peng Shu, Yi Wu, Zhaoqiao Liu, Yongfei Jian, Mengbo Wu, Yikui Wang, Zhengguo Li, Julien Pirrello, Mondher Bouzayen, Wei Deng, Yiguo Hong, Mingchun Liu

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引用次数: 0

摘要

植物激素乙烯在果实抵御灰霉病菌侵袭的过程中起着至关重要的作用，但人们对其基本机制仍然知之甚少。在这里，我们发现乙烯响应因子 SlERF.C1 是番茄果实中乙烯介导的防御灰霉病的关键调节因子，同时不会影响果实的成熟。SlERF.C1的敲除增加了果实对B. cinerea的易感性，但对成熟过程没有影响，而过表达则增强了抗性。RNA-Seq、转录激活试验、EMSA 和 ChIP-qPCR 结果表明，SlERF.C1 通过与 PR 基因的启动子结合激活了 PR 基因的转录。此外，SlERF.C1还与丝裂原活化蛋白激酶SlMPK8相互作用，使SlMPK8在Ser174残基上磷酸化SlERF.C1，从而提高其转录活性。敲除 SlMPK8 会增加果实对 B. cinerea 的易感性，而过表达会增强抗性，但不影响成熟。此外，SlMPK8-KO 和 SlERF.C1-OE 株系之间的遗传杂交降低了 SlERF.C1-OE 果实对琴叶枯病的抗性。此外，B. cinerea 感染会诱导乙烯的产生，而乙烯的产生又会触发 SlMPK8 的转录并增强 SlERF.C1 的磷酸化。总之，我们的研究结果揭示了 "乙烯-MPK8-ERF.C1-PR "模块在抗灰霉病中的调控机制，并为操纵水果灰霉病的发生提供了新的见解。

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Ethylene-MPK8-ERF.C1-PR module confers resistance against Botrytis cinerea in tomato fruit without compromising ripening

The plant hormone ethylene plays a critical role in fruit defense against Botrytis cinerea attack, but the underlying mechanisms remain poorly understood. Here, we showed that ethylene response factor SlERF.C1 acts as a key regulator to trigger the ethylene-mediated defense against B. cinerea in tomato fruits without compromising ripening.
Knockout of SlERF.C1 increased fruit susceptibility to B. cinerea with no effect on ripening process, while overexpression enhanced resistance. RNA-Seq, transactivation assays, EMSA and ChIP-qPCR results indicated that SlERF.C1 activated the transcription of PR genes by binding to their promoters.
Moreover, SlERF.C1 interacted with the mitogen-activated protein kinase SlMPK8 which allowed SlMPK8 to phosphorylate SlERF.C1 at the Ser¹⁷⁴ residue and increases its transcriptional activity. Knocking out of SlMPK8 increased fruit susceptibility to B. cinerea, whereas overexpression enhanced resistance without affecting ripening. Furthermore, genetic crosses between SlMPK8-KO and SlERF.C1-OE lines reduced the resistance to B. cinerea attack in SlERF.C1-OE fruits. In addition, B. cinerea infection induced ethylene production which in turn triggered SlMPK8 transcription and enhanced the phosphorylation of SlERF.C1.
Overall, our findings reveal the regulatory mechanism of the ‘Ethylene-MPK8-ERF.C1-PR’ module in resistance against B. cinerea and provide new insight into the manipulation of gray mold disease in fruits.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.