Tao Yang, Kingwai Lai, Yang Yu, Zongzhen Liao, Ran Cai, Xiaozheng Yu, Wensheng Li
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引用次数: 0
摘要
已知神经肽 Y 直接或间接参与免疫调节。NPY 的免疫效应包括免疫细胞转运、辅助性 T 细胞分化、细胞因子分泌、染色和杀伤细胞活性、吞噬作用和活性氧的产生。在这项研究中，我们研究了合成 NPY 对大口鲈鱼幼体的免疫保护作用。首次探讨了注射 NPY 对大口鲈鱼的剂量和时间效应，并在注射 NPY 后分别对大口鲈鱼幼体进行了 Poly I:C 和 LPS 感染试验。结果表明，NPY能抑制头肾、脾、脑中il-1β、tgf-β、ifn-γ等免疫因子的表达，从而减轻炎症反应，缓解感染早期强烈炎症反应引起的免疫应激。同时，注射NPY能改善感染引起的肠道组织损伤。这项研究为保护幼鱼和提高其先天免疫力提供了一种新方法。
Effects of neuropeptide Y on the immune-protection and intestinal tract of juvenile Micropterus salmoides
Neuropeptide Y is known to be directly or indirectly involved in immune regulation. The immune effects of NPY include immune cell transport, helper T cell differentiation, cytokine secretion, staining and killer cell activity, phagocytosis and production of reactive oxygen species. In this study, we investigated the immunoprotective effect of synthetic NPY on largemouth bass larvae. For the first time, the dose and time effects of NPY injection on largemouth bass was explored, and then Poly I:C and LPS infection was carried out in juvenile largemouth bass, respectively, after the injection of NPY. The results showed that NPY could reduce the inflammatory response by inhibiting the expression of il-1β, tgf-β, ifn-γ and other immune factors in head kidney, spleen and brain, and alleviate the immune stress caused by strong inflammatory response in the early stage of infection. Meanwhile, NPY injection ameliorated the intestinal tissue damage caused by infection. This study provides a new way to protect juvenile fish and improve its innate immunity.
期刊介绍：
General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.
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