锂负载 GelMA 磷酸盐玻璃纤维构建体:对星形胶质细胞反应的影响

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Zalike Keskin-Erdogan, Nandin Mandakhbayar, Gang Shi Jin, Yu-Meng Li, David Y. S. Chau, Richard M. Day, Hae-Won Kim, Jonathan C. Knowles
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引用次数: 0

摘要

在组织工程学中,不同的生物材料凭借自身的优势进行组合,并与其他成分进行功能化处理,以提高整体材料的性能,这种做法由来已久。生物材料,尤其是水凝胶平台,已显示出在神经组织工程应用中输送药物、生长因子、神经营养因子等化合物以及细胞的巨大潜力。在中枢神经系统中,星形胶质细胞的反应性和胶质疤痕的形成是神经和功能恢复所面临的重大而复杂的挑战。本研究开发了基于 GelMA 水凝胶的组织构建物,并将其与两种不同配方的磷酸盐玻璃纤维(PGFs)(含 Fe3+ 或 Ti2+ 氧化物)相结合,以施加物理和机械线索来调节星形胶质细胞的行为。本研究还旨在探讨锂负载 GelMA-PGFs 水凝胶在减轻星形胶质细胞反应性和胶质瘢痕形成方面的效果,为神经组织工程应用提供新的视角。引入锂的理由是,锂对精神疾病的治疗效果早已得到证实,而且具有神经保护和明显的抗炎特性。我们在体外测定了锂和 LPS 对原代大鼠星形胶质细胞的最佳浓度。此外,还进行了 qPCR 基因表达分析,分析了在含锂和不含锂的情况下,将原代星形胶质细胞三维培养到 GelMA 和 GelMA-PGFs 水凝胶中,并在体外用 LPS 刺激星形胶质细胞反应的情况下,GFAP 和 IL-6 标记的基因表达情况。结果表明,将生物活性磷酸盐基玻璃纤维和锂负载结合到 GelMA 结构中可能会影响 GFAP 的表达和早期 IL-6 的表达。此外,GelMA-PGFs(铁)构建物在调节神经胶质瘢痕方面的表现优于 GFAP 调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lithium-loaded GelMA-Phosphate glass fibre constructs: Implications for astrocyte response

Lithium-loaded GelMA-Phosphate glass fibre constructs: Implications for astrocyte response

Lithium-loaded GelMA-Phosphate glass fibre constructs: Implications for astrocyte response

Combinations of different biomaterials with their own advantages as well as functionalization with other components have long been implemented in tissue engineering to improve the performance of the overall material. Biomaterials, particularly hydrogel platforms, have shown great potential for delivering compounds such as drugs, growth factors, and neurotrophic factors, as well as cells, in neural tissue engineering applications. In central the nervous system, astrocyte reactivity and glial scar formation are significant and complex challenges to tackle for neural and functional recovery. GelMA hydrogel-based tissue constructs have been developed in this study and combined with two different formulations of phosphate glass fibers (PGFs) (with Fe3+ or Ti2+ oxide) to impose physical and mechanical cues for modulating astrocyte cell behavior. This study was also aimed at investigating the effects of lithium-loaded GelMA-PGFs hydrogels in alleviating astrocyte reactivity and glial scar formation offering novel perspectives for neural tissue engineering applications. The rationale behind introducing lithium is driven by its long-proven therapeutic benefits in mental disorders, and neuroprotective and pronounced anti-inflammatory properties. The optimal concentrations of lithium and LPS were determined in vitro on primary rat astrocytes. Furthermore, qPCR was conducted for gene expression analysis of GFAP and IL-6 markers on primary astrocytes cultured 3D into GelMA and GelMA-PGFs hydrogels with and without lithium and in vitro stimulated with LPS for astrocyte reactivity. The results suggest that the combination of bioactive phosphate-based glass fibers and lithium loading into GelMA structures may impact GFAP expression and early IL-6 expression. Furthermore, GelMA-PGFs (Fe) constructs have shown improved performance in modulating glial scarring over GFAP regulation.

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来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
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