利用月经盘对女性下生殖道免疫细胞进行采样的无创方法

Q3 Medicine
M Quinn Peters, Eva Domenjo-Vila, Marc Carlson, Blair Armistead, Paul T Edlefsen, Melanie Gasper, Smritee Dabee, Christopher Whidbey, Heather B Jaspan, Martin Prlic, Whitney E Harrington
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引用次数: 0

摘要

人类女性生殖道(FGT)中的 T 细胞是易受感染和免受感染(包括 HIV 和其他性传播感染)的关键介质。我们亟需进一步了解 FGT 中 T 细胞群的分布和活化情况,但目前的采样方法需要医疗保健提供者,而且费用昂贵,限制了对这些细胞群进行纵向研究的能力。为了应对这些挑战,我们开发了一种利用一次性月经盘从 FGT 提取免疫细胞样本的方法,这种方法无创、可自行应用且成本低廉。为了证明这种方法的可重复性,我们使用月经盘连续三天对健康育龄个体的宫颈阴道液进行采样。我们对宫颈阴道液进行了处理,以检测宫颈阴道细胞,并使用高参数流式细胞术鉴定免疫群体的特征。我们发现了大量活的 CD45+ 白细胞,以及不同的 T 细胞和 B 细胞群。在 T 细胞区系中,T 细胞亚群的活化和抑制状态与之前利用现有方法对 FGT 进行的研究一致,包括组织驻留和迁移群的鉴定。此外,T细胞群体结构在个体内部不同天数之间高度一致,但在个体之间存在差异。我们利用月经盘对 FGT 中的免疫细胞进行采样的方法将减少参与研究的障碍,并有助于在未来的研究中进行纵向采样。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Noninvasive Method to Sample Immune Cells in the Lower Female Genital Tract Using Menstrual Discs.

T cells in the human female genital tract (FGT) are key mediators of susceptibility to and protection from infection, including HIV and other sexually transmitted infections. There is a critical need for increased understanding of the distribution and activation of T cell populations in the FGT, but current sampling methods require a healthcare provider and are expensive, limiting the ability to study these populations longitudinally. To address these challenges, we have developed a method to sample immune cells from the FGT utilizing disposable menstrual discs which are noninvasive, self-applied, and low in cost. To demonstrate reproducibility, we sampled the cervicovaginal fluid of healthy, reproductive-aged individuals using menstrual discs across 3 sequential days. Cervicovaginal fluid was processed for cervicovaginal cells, and high-parameter flow cytometry was used to characterize immune populations. We identified large numbers of live, CD45+ leukocytes, as well as distinct populations of T cells and B cells. Within the T cell compartment, activation and suppression status of T cell subsets were consistent with previous studies of the FGT utilizing current approaches, including identification of both tissue-resident and migratory populations. In addition, the T cell population structure was highly conserved across days within individuals but divergent across individuals. Our approach to sample immune cells in the FGT with menstrual discs will decrease barriers to participation and empower longitudinal sampling in future research studies.

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来源期刊
CiteScore
3.70
自引率
0.00%
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审稿时长
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