{"title":"α-蒎烯对 AGS 胃癌细胞的抗癌作用","authors":"Eun-Ji Han, Eun-Young Choi, Su-Ji Jeon, Jun-Mo Moon, Sang-Woo Lee, Jae-Han Lee, Gi-Hwan Jung, So-Hee Han, Soo-Hyun Jung, Myeon-Sik Yang, Ji-Youn Jung","doi":"10.1089/jmf.2023.K.0267","DOIUrl":null,"url":null,"abstract":"<p><p>Gastric cancer is the fifth most common cancer globally and the third leading cause of cancer-related mortality. Existing treatment strategies for gastric cancer often present numerous side effects. Consequently, recent studies have shifted toward devising new treatments grounded in safer natural substances. <i>α</i>-Pinene, a natural terpene found in the essential oils of various plants, such as <i>Lavender angustifolia</i> and <i>Satureja myrtifolia</i>, displays antioxidant, antibiotic, and anticancer properties. Yet, its impact on gastric cancer remains unexplored. This research assessed the effects of <i>α</i>-pinene <i>in vitro</i> using a human gastric adenocarcinoma cell-line (AGS) human gastric cancer cells and <i>in vivo</i> via a xenograft mouse model. The survival rate of AGS cells treated with <i>α</i>-pinene was notably lower than that of the control group, as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. This decline in cell viability was linked to apoptosis, as verified by 4',6-diamidino-2-phenylindole and annexin V/propidium iodide staining. The <i>α</i>-pinene-treated group exhibited elevated cleaved-poly (ADP-ribose) polymerase and B cell lymphoma 2 (Bcl-2)-associated X (Bax) levels and reduced Bcl-2 levels compared with the control levels. Moreover, <i>α</i>-pinene triggered the activation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 within the mitogen-activated protein kinase (MAPK) pathway. In the xenograft mouse model, <i>α</i>-pinene induced apoptosis through the MAPK pathway, devoid of toxicity. These findings position <i>α</i>-pinene as a promising natural therapeutic for gastric cancer.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anticancer Effects of <i>α</i>-Pinene in AGS Gastric Cancer Cells.\",\"authors\":\"Eun-Ji Han, Eun-Young Choi, Su-Ji Jeon, Jun-Mo Moon, Sang-Woo Lee, Jae-Han Lee, Gi-Hwan Jung, So-Hee Han, Soo-Hyun Jung, Myeon-Sik Yang, Ji-Youn Jung\",\"doi\":\"10.1089/jmf.2023.K.0267\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gastric cancer is the fifth most common cancer globally and the third leading cause of cancer-related mortality. Existing treatment strategies for gastric cancer often present numerous side effects. Consequently, recent studies have shifted toward devising new treatments grounded in safer natural substances. <i>α</i>-Pinene, a natural terpene found in the essential oils of various plants, such as <i>Lavender angustifolia</i> and <i>Satureja myrtifolia</i>, displays antioxidant, antibiotic, and anticancer properties. Yet, its impact on gastric cancer remains unexplored. This research assessed the effects of <i>α</i>-pinene <i>in vitro</i> using a human gastric adenocarcinoma cell-line (AGS) human gastric cancer cells and <i>in vivo</i> via a xenograft mouse model. The survival rate of AGS cells treated with <i>α</i>-pinene was notably lower than that of the control group, as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. This decline in cell viability was linked to apoptosis, as verified by 4',6-diamidino-2-phenylindole and annexin V/propidium iodide staining. The <i>α</i>-pinene-treated group exhibited elevated cleaved-poly (ADP-ribose) polymerase and B cell lymphoma 2 (Bcl-2)-associated X (Bax) levels and reduced Bcl-2 levels compared with the control levels. Moreover, <i>α</i>-pinene triggered the activation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 within the mitogen-activated protein kinase (MAPK) pathway. In the xenograft mouse model, <i>α</i>-pinene induced apoptosis through the MAPK pathway, devoid of toxicity. These findings position <i>α</i>-pinene as a promising natural therapeutic for gastric cancer.</p>\",\"PeriodicalId\":16440,\"journal\":{\"name\":\"Journal of medicinal food\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of medicinal food\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1089/jmf.2023.K.0267\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of medicinal food","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/jmf.2023.K.0267","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/22 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Anticancer Effects of α-Pinene in AGS Gastric Cancer Cells.
Gastric cancer is the fifth most common cancer globally and the third leading cause of cancer-related mortality. Existing treatment strategies for gastric cancer often present numerous side effects. Consequently, recent studies have shifted toward devising new treatments grounded in safer natural substances. α-Pinene, a natural terpene found in the essential oils of various plants, such as Lavender angustifolia and Satureja myrtifolia, displays antioxidant, antibiotic, and anticancer properties. Yet, its impact on gastric cancer remains unexplored. This research assessed the effects of α-pinene in vitro using a human gastric adenocarcinoma cell-line (AGS) human gastric cancer cells and in vivo via a xenograft mouse model. The survival rate of AGS cells treated with α-pinene was notably lower than that of the control group, as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. This decline in cell viability was linked to apoptosis, as verified by 4',6-diamidino-2-phenylindole and annexin V/propidium iodide staining. The α-pinene-treated group exhibited elevated cleaved-poly (ADP-ribose) polymerase and B cell lymphoma 2 (Bcl-2)-associated X (Bax) levels and reduced Bcl-2 levels compared with the control levels. Moreover, α-pinene triggered the activation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 within the mitogen-activated protein kinase (MAPK) pathway. In the xenograft mouse model, α-pinene induced apoptosis through the MAPK pathway, devoid of toxicity. These findings position α-pinene as a promising natural therapeutic for gastric cancer.
期刊介绍:
Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.