Ayomide H. Labulo, Oyinade A. David, Augustine D. Terna, Timileyin P. Omotosho, Nicholas S. Tanko, Ibrahim Hassan, Bosede R. Oluwole, Adeyinka Odebode
{"title":"干旱胁迫下绿色合成银纳米粒子和褪黑素对单叶洋金花生理生化活性的调节作用","authors":"Ayomide H. Labulo, Oyinade A. David, Augustine D. Terna, Timileyin P. Omotosho, Nicholas S. Tanko, Ibrahim Hassan, Bosede R. Oluwole, Adeyinka Odebode","doi":"10.1007/s11816-024-00887-4","DOIUrl":null,"url":null,"abstract":"<p>The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed <i>E. uniflora</i>. Sterilized seeds of <i>E. uniflora</i> were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup>. The ML-AgNO<sub>3</sub> showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Modulation of physiological and biochemical activities of Eugenia uniflora by green-synthesized silver nanoparticle and melatonin under drought stress\",\"authors\":\"Ayomide H. Labulo, Oyinade A. David, Augustine D. Terna, Timileyin P. Omotosho, Nicholas S. Tanko, Ibrahim Hassan, Bosede R. Oluwole, Adeyinka Odebode\",\"doi\":\"10.1007/s11816-024-00887-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed <i>E. uniflora</i>. Sterilized seeds of <i>E. uniflora</i> were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup>. The ML-AgNO<sub>3</sub> showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress.</p>\",\"PeriodicalId\":20216,\"journal\":{\"name\":\"Plant Biotechnology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-02-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology Reports\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11816-024-00887-4\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Reports","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11816-024-00887-4","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Modulation of physiological and biochemical activities of Eugenia uniflora by green-synthesized silver nanoparticle and melatonin under drought stress
The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed E. uniflora. Sterilized seeds of E. uniflora were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H2O2 and O2−. The ML-AgNO3 showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress.
期刊介绍:
Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.