Jianxin Liang , Imtiaz Ul Hassan , Man Yee Cheung , Lei Feng , Yi-jyun Lin , Qi Long , Chengdong Wang , Yuyue Ding , Ziqing Wang , Yuan Zhang , Yulong Li , Donghao Guo , Xiaofang Guo , Thomas Chi Bun Wong , Muhammad Kaleem Samma , Zixin Rong , Xufeng Qi , Dongqing Cai , Sai-Ming Ngai , Hui Zhao
{"title":"心脏发育过程中转录因子 Sox18 的机制研究","authors":"Jianxin Liang , Imtiaz Ul Hassan , Man Yee Cheung , Lei Feng , Yi-jyun Lin , Qi Long , Chengdong Wang , Yuyue Ding , Ziqing Wang , Yuan Zhang , Yulong Li , Donghao Guo , Xiaofang Guo , Thomas Chi Bun Wong , Muhammad Kaleem Samma , Zixin Rong , Xufeng Qi , Dongqing Cai , Sai-Ming Ngai , Hui Zhao","doi":"10.1016/j.ygcen.2024.114472","DOIUrl":null,"url":null,"abstract":"<div><p>Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of <em>sox18</em> in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that <em>sox18</em> is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of <em>Xenopus tropicalis</em> embryo. Overexpression of <em>sox18</em> in the <em>X. tropicalis</em> embryos causes heart edema, while loss-of-function of <em>sox18</em> can change the signal of developmental heart marker <em>gata4</em> at different stages, suggesting that <em>sox18</em> plays an essential role in the development of the heart. Knockdown of <em>SOX18</em> in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates <em>islet1</em> and <em>tbx3</em>, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanistic study of transcription factor Sox18 during heart development\",\"authors\":\"Jianxin Liang , Imtiaz Ul Hassan , Man Yee Cheung , Lei Feng , Yi-jyun Lin , Qi Long , Chengdong Wang , Yuyue Ding , Ziqing Wang , Yuan Zhang , Yulong Li , Donghao Guo , Xiaofang Guo , Thomas Chi Bun Wong , Muhammad Kaleem Samma , Zixin Rong , Xufeng Qi , Dongqing Cai , Sai-Ming Ngai , Hui Zhao\",\"doi\":\"10.1016/j.ygcen.2024.114472\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of <em>sox18</em> in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that <em>sox18</em> is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of <em>Xenopus tropicalis</em> embryo. Overexpression of <em>sox18</em> in the <em>X. tropicalis</em> embryos causes heart edema, while loss-of-function of <em>sox18</em> can change the signal of developmental heart marker <em>gata4</em> at different stages, suggesting that <em>sox18</em> plays an essential role in the development of the heart. Knockdown of <em>SOX18</em> in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates <em>islet1</em> and <em>tbx3</em>, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling.</p></div>\",\"PeriodicalId\":12582,\"journal\":{\"name\":\"General and comparative endocrinology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-02-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"General and comparative endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0016648024000327\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"General and comparative endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0016648024000327","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Mechanistic study of transcription factor Sox18 during heart development
Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling.
期刊介绍：
General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.
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