铜通过牛巨噬细胞中的 NF-кB 通路促进 LPS 诱导的炎症反应

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biological Trace Element Research Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI:10.1007/s12011-024-04107-6
Hongrui Guo, Lin Jing, Chenglong Xia, Yanqiu Zhu, Yue Xie, Xiaoping Ma, Jing Fang, Zhisheng Wang, Zhicai Zuo
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引用次数: 0

摘要

炎症是一个复杂的生理过程,能够清除病原体和修复受损组织。有报道称,炎症患者的血清铜浓度会升高,但铜在炎症反应中的作用仍不清楚。本研究使用牛巨噬细胞建立脂多糖(LPS)诱导的炎症模型。研究共分五组:一组用 LPS(100 ng/ml)处理,一组在 LPS 刺激后用铜螯合剂(四硫代钼酸盐,TTM)(20 μmol)或 CuSO4(25 μmol 或 50 μmol)处理,另一组为对照组。经 LPS 处理后,巨噬细胞中的铜浓度增加。TTM 降低了促炎因子(IL-1β、TNF-α、IL-6、iNOS 和 COX-2)的 mRNA 表达,而铜补充剂则增加了这些因子的表达。与对照组相比,TTM 组和铜组的 TLP4 和 MyD88 蛋白水平均有所提高。然而,TTM 处理降低了 p-p65,增加了 IкB-α,而铜补充剂则显示出相反的结果。此外,TTM 处理组牛巨噬细胞的吞噬和迁移能力下降,而铜处理组则上升。上述结果表明,铜可促进 LPS 诱导的牛巨噬细胞炎症反应,通过激活 NF-кB 通路促进促炎因子,并提高吞噬能力和迁移能力。我们的研究为牛的炎症提供了一种可能的靶向疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Copper Promotes LPS-Induced Inflammation via the NF-кB Pathway in Bovine Macrophages.

Inflammation is a complex physiological process that enables the clearance of pathogens and repairing damaged tissues. Elevated serum copper concentration has been reported in cases of inflammation, but the role of copper in inflammatory responses remains unclear. This study used bovine macrophages to establish lipopolysaccharide (LPS)-induced inflammation model. There were five groups in the study: a group treated with LPS (100 ng/ml), a group treated with either copper chelator (tetrathiomolybdate, TTM) (20 μmol) or CuSO4 (25 μmol or 50 μmol) after LPS stimulation, and a control group. Copper concentrations increased in macrophages after the LPS treatment. TTM decreased mRNA expression of pro-inflammatory factors (IL-1β, TNF-α, IL-6, iNOS, and COX-2), whereas copper supplement increased them. Compared to the control group, TLP4 and MyD88 protein levels were increased in the TTM and copper groups. However, TTM treatment decreased p-p65 and increased IкB-α while the copper supplement showed reversed results. In addition, the phagocytosis and migration of bovine macrophages decreased in the TTM treatment group while increased in the copper treatment groups. Results mentioned above indicated that copper could promote the LPS-induced inflammatory response in bovine macrophages, promote pro-inflammatory factors by activating the NF-кB pathway, and increase phagocytosis capacity and migration. Our study provides a possible targeted therapy for bovine inflammation.

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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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