从山茶花中提取的没食子酸甲酯可降低与法定量感应相关的毒力和生物膜的形成，从而抑制嗜水气单胞菌。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2024-02-19 DOI:10.1080/08927014.2024.2316611

Huan Jiang, Zhennan Wang, Ai-Qun Jia

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引用次数: 0

摘要

嗜水气单胞菌（Aeromonas hydrophila）是一种革兰氏阴性的人畜共患细菌，在养鱼业和免疫力低下的病人中造成很高的死亡率。本研究旨在从山茶花中提取没食子酸甲酯（MG），并评估其作为法定量感应抑制剂（QSI）对嗜水气单胞菌 SHAe 115 的潜在作用。MG 可减少与 QS 相关的毒力因子，包括溶血、蛋白酶和脂肪酶，同时影响游动性和生物膜的形成。此外，MG 下调了正调控基因（hyR、fleQ），上调了负调控基因（litR、fleN）。这凸显了 MG 作为一种强效 QSI 对嗜水甲藻 SHAe 115 的前景，有助于推进水产养殖和人类健康中的抗感染策略。

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Methyl gallate from Camellia nitidissima Chi flowers reduces quorum sensing related virulence and biofilm formation against Aeromonas hydrophila.

Aeromonas hydrophila, a Gram-negative zoonotic bacterium, causes high mortality in fish farming and immunocompromised patients. This study aimed to extract methyl gallate (MG) from the flowers of Camellia nitidissima Chi and evaluate its potential as a quorum sensing inhibitor (QSI) against Aeromonas hydrophila SHAe 115. MG reduced QS-associated virulence factors, including hemolysis, protease, and lipase, while impairing swimming motility and biofilm formation. Additionally, MG down-regulated positive regulatory genes (ahyR, fleQ) and up-regulated negative regulators (litR, fleN). This highlights MG's promise as a potent QSI for A. hydrophila SHAe 115, advancing strategies against infections in aquaculture and human health.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464