{"title":"万古霉素的氚标记及其在纳米金刚石上的吸附研究","authors":"Т. Shen, M. G. Chernysheva, G. A. Badun","doi":"10.1134/S1066362223060103","DOIUrl":null,"url":null,"abstract":"<p> Procedure of tritium labeling vancomycin synthesis using tritium thermal activation method was developed. The influence of target mass on the specific and total radioactivity was revealed. [<sup>3</sup>H]Vancomycin was used for studying its equilibrium adsorption on nanodiamonds as well as its amount that tightly bonded to surface and did not remove with water. It was found that vancomycin is adsorbed from aqueous solution via strong bonding to nanodiamonds and is not washed out with water. Application of 0.028 M phosphate buffer (pH 6.7 and 2.7) increased the equilibrium vancomycin adsorption about one and a half times, while vancomycin amount in the adsorption complex with nanodiamonds after washing with water was significantly reduced. Such behavior of vancomycin is due to the presence of phosphate-ions that contribute to vancomycin adsorption but are washed out with water. Molecular mechanics simulation allows the suggestion that the formation of multiple hydrogen bonds is required for generation of a durable adsorption complex of vancomycin with nanodiamonds.</p>","PeriodicalId":747,"journal":{"name":"Radiochemistry","volume":"65 6","pages":"690 - 698"},"PeriodicalIF":0.9000,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tritium Labeling Vancomycin and Studying Its Adsorption on Nanodiamonds\",\"authors\":\"Т. Shen, M. G. Chernysheva, G. A. Badun\",\"doi\":\"10.1134/S1066362223060103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p> Procedure of tritium labeling vancomycin synthesis using tritium thermal activation method was developed. The influence of target mass on the specific and total radioactivity was revealed. [<sup>3</sup>H]Vancomycin was used for studying its equilibrium adsorption on nanodiamonds as well as its amount that tightly bonded to surface and did not remove with water. It was found that vancomycin is adsorbed from aqueous solution via strong bonding to nanodiamonds and is not washed out with water. Application of 0.028 M phosphate buffer (pH 6.7 and 2.7) increased the equilibrium vancomycin adsorption about one and a half times, while vancomycin amount in the adsorption complex with nanodiamonds after washing with water was significantly reduced. Such behavior of vancomycin is due to the presence of phosphate-ions that contribute to vancomycin adsorption but are washed out with water. Molecular mechanics simulation allows the suggestion that the formation of multiple hydrogen bonds is required for generation of a durable adsorption complex of vancomycin with nanodiamonds.</p>\",\"PeriodicalId\":747,\"journal\":{\"name\":\"Radiochemistry\",\"volume\":\"65 6\",\"pages\":\"690 - 698\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-02-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Radiochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1066362223060103\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiochemistry","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S1066362223060103","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Tritium Labeling Vancomycin and Studying Its Adsorption on Nanodiamonds
Procedure of tritium labeling vancomycin synthesis using tritium thermal activation method was developed. The influence of target mass on the specific and total radioactivity was revealed. [3H]Vancomycin was used for studying its equilibrium adsorption on nanodiamonds as well as its amount that tightly bonded to surface and did not remove with water. It was found that vancomycin is adsorbed from aqueous solution via strong bonding to nanodiamonds and is not washed out with water. Application of 0.028 M phosphate buffer (pH 6.7 and 2.7) increased the equilibrium vancomycin adsorption about one and a half times, while vancomycin amount in the adsorption complex with nanodiamonds after washing with water was significantly reduced. Such behavior of vancomycin is due to the presence of phosphate-ions that contribute to vancomycin adsorption but are washed out with water. Molecular mechanics simulation allows the suggestion that the formation of multiple hydrogen bonds is required for generation of a durable adsorption complex of vancomycin with nanodiamonds.
期刊介绍：
Radiochemistry is a journal that covers the theoretical and applied aspects of radiochemistry, including basic nuclear physical properties of radionuclides; chemistry of radioactive elements and their compounds; the occurrence and behavior of natural and artificial radionuclides in the environment; nuclear fuel cycle; radiochemical analysis methods and devices; production and isolation of radionuclides, synthesis of labeled compounds, new applications of radioactive tracers; radiochemical aspects of nuclear medicine; radiation chemistry and after-effects of nuclear transformations.
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