益生菌乳酸杆菌通过提高大脑蘑菇体神经元的乳酸脱氢酶水平改善果蝇的记忆。

IF 12.2 1区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-02-19 DOI:10.1080/19490976.2024.2316533
Shuk-Man Ho, Wan-Hua Tsai, Chih-Ho Lai, Meng-Hsuan Chiang, Wang-Po Lee, Hui-Yu Wu, Pei-Yi Bai, Tony Wu, Chia-Lin Wu
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引用次数: 0

摘要

益生菌是活的微生物,能为宿主带来潜在的益处,偶尔也能影响宿主的行为反应。然而,益生菌影响宿主行为的详细机制和潜在的生物效应仍不清楚。乳酸菌,特别是乳酸杆菌是已知的益生菌。黑腹果蝇(俗称果蝇)是在转化研究中调查宿主与肠道微生物群之间相互作用的成熟模式生物。在这里,我们发现,给果蝇喂食 5 天的嗜酸乳杆菌(称为 GMNL-185)或鼠李糖乳杆菌(称为 GMNL-680)可增强其嗅觉联想记忆。此外,GMNL-185 和 GMNL-680 的联合饮食对记忆功能有协同作用。活体脑成像显示,混合喂食GMNL-185和GMNL-680的果蝇蘑菇体β叶和γ叶对训练气味的钙反应明显增加。定量逆转录聚合酶链反应(qRT-PCR)和全贴片脑免疫组化显示,混合喂养后蝇蛆脑内乳酸脱氢酶(LDH)的表达明显上调。值得注意的是,基因敲除神经元(特别是蘑菇体)中的 Ldh 能改善 GMNL-185 和 GMNL-680 混合喂养对记忆改善的有益影响。总之,我们的研究结果表明,补充嗜酸乳杆菌和鼠李糖杆菌可通过提高大脑 LDH 水平来增强苍蝇的记忆功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Probiotic Lactobacillus spp. improves Drosophila memory by increasing lactate dehydrogenase levels in the brain mushroom body neurons.

Probiotics are live microorganisms that offer potential benefits to their hosts and can occasionally influence behavioral responses. However, the detailed mechanisms by which probiotics affect the behavior of their hosts and the underlying biogenic effects remain unclear. Lactic acid bacteria, specifically Lactobacillus spp. are known probiotics. Drosophila melanogaster, commonly known as the fruit fly, is a well-established model organism for investigating the interaction between the host and gut microbiota in translational research. Herein, we showed that 5-day administration of Lactobacillus acidophilus (termed GMNL-185) or Lacticaseibacillus rhamnosus (termed GMNL-680) enhances olfactory-associative memory in Drosophila. Moreover, a combined diet of GMNL-185 and GMNL-680 demonstrated synergistic effects on memory functions. Live brain imaging revealed a significant increase in calcium responses to the training odor in the mushroom body β and γ lobes of flies that underwent mixed feeding with GMNL-185 and GMNL-680. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and whole-mount brain immunohistochemistry revealed significant upregulation of lactate dehydrogenase (LDH) expression in the fly brain following the mixed feeding. Notably, the genetic knockdown of Ldh in neurons, specifically in mushroom body, ameliorated the beneficial effects of mixed feeding with GMNL-185 and GMNL-680 on memory improvement. Altogether, our results demonstrate that supplementation with L. acidophilus and L. rhamnosus enhances memory functions in flies by increasing brain LDH levels.

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来源期刊
Gut Microbes
Gut Microbes Medicine-Microbiology (medical)
CiteScore
18.20
自引率
3.30%
发文量
196
审稿时长
10 weeks
期刊介绍: The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more. Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.
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