Carolina Netto de Oliveira da Cunha , Stéfani Rodeghiero Collares , Débora Carvalho Rodrigues , Débora Liliane Walcher , Micaele Quintana de Moura , Lourdes Helena Rodrigues Martins , Gabriel Baracy Klafke , Jean Lucas de Oliveira Arias , Rodolfo Carapelli , João Batista do Santos Espinelli Junior , Carlos James Scaini , Luciana Farias da Costa de Avila
{"title":"嗜酸乳杆菌 ATCC 4356 上清液对犬弓形虫的杀幼虫作用","authors":"Carolina Netto de Oliveira da Cunha , Stéfani Rodeghiero Collares , Débora Carvalho Rodrigues , Débora Liliane Walcher , Micaele Quintana de Moura , Lourdes Helena Rodrigues Martins , Gabriel Baracy Klafke , Jean Lucas de Oliveira Arias , Rodolfo Carapelli , João Batista do Santos Espinelli Junior , Carlos James Scaini , Luciana Farias da Costa de Avila","doi":"10.1016/j.exppara.2024.108720","DOIUrl":null,"url":null,"abstract":"<div><p>Human toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with <em>Lactobacillus acidophilus</em> ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of <em>Toxocara canis</em> larvae <em>in vitro</em>. The present study aimed to investigate the characteristics of the CFS of <em>L. acidophilus</em> ATCC 4356, which may be involved in its larvicidal effects on <em>T. canis</em>. <em>L. acidophilus</em> ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quantified by high performance liquid chromatography (HPLC). The levels of pH and H<sub>2</sub>O<sub>2</sub> were also analyzed. To assess the larvicidal effect of the CFS, this was tested pure and diluted (1:2 to 1:128) on <em>T. canis</em> larvae. High concentrations of lactic and acetic acids were detected in the CFS. The acidity of the pure CFS was observed at pH 3.8, remaining acidic at dilutions of 1:2 to 1:16. Regarding the <em>in vitro</em> larvicidal effect, 100% death of <em>T. canis</em> larvae was observed using the pure CFS and 1:2 dilution. Based on these results, it can be inferred that the presence of higher concentrations of organic acids and low pH of the medium contributed to the larvicidal activity of the CFS of <em>L. acidophilus</em> ATCC 4356. In addition, the maintenance of the larvicidal effect, even after dilution, suggests a greater chance of the larvicidal effect of this CFS against <em>T. canis in vivo</em>.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The larvicidal effect of the supernatant of Lactobacillus acidophilus ATCC 4356 on Toxocara canis\",\"authors\":\"Carolina Netto de Oliveira da Cunha , Stéfani Rodeghiero Collares , Débora Carvalho Rodrigues , Débora Liliane Walcher , Micaele Quintana de Moura , Lourdes Helena Rodrigues Martins , Gabriel Baracy Klafke , Jean Lucas de Oliveira Arias , Rodolfo Carapelli , João Batista do Santos Espinelli Junior , Carlos James Scaini , Luciana Farias da Costa de Avila\",\"doi\":\"10.1016/j.exppara.2024.108720\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Human toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with <em>Lactobacillus acidophilus</em> ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of <em>Toxocara canis</em> larvae <em>in vitro</em>. The present study aimed to investigate the characteristics of the CFS of <em>L. acidophilus</em> ATCC 4356, which may be involved in its larvicidal effects on <em>T. canis</em>. <em>L. acidophilus</em> ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quantified by high performance liquid chromatography (HPLC). The levels of pH and H<sub>2</sub>O<sub>2</sub> were also analyzed. To assess the larvicidal effect of the CFS, this was tested pure and diluted (1:2 to 1:128) on <em>T. canis</em> larvae. High concentrations of lactic and acetic acids were detected in the CFS. The acidity of the pure CFS was observed at pH 3.8, remaining acidic at dilutions of 1:2 to 1:16. Regarding the <em>in vitro</em> larvicidal effect, 100% death of <em>T. canis</em> larvae was observed using the pure CFS and 1:2 dilution. Based on these results, it can be inferred that the presence of higher concentrations of organic acids and low pH of the medium contributed to the larvicidal activity of the CFS of <em>L. acidophilus</em> ATCC 4356. In addition, the maintenance of the larvicidal effect, even after dilution, suggests a greater chance of the larvicidal effect of this CFS against <em>T. canis in vivo</em>.</p></div>\",\"PeriodicalId\":12117,\"journal\":{\"name\":\"Experimental parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-02-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014489424000237\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489424000237","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
The larvicidal effect of the supernatant of Lactobacillus acidophilus ATCC 4356 on Toxocara canis
Human toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with Lactobacillus acidophilus ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of Toxocara canis larvae in vitro. The present study aimed to investigate the characteristics of the CFS of L. acidophilus ATCC 4356, which may be involved in its larvicidal effects on T. canis. L. acidophilus ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quantified by high performance liquid chromatography (HPLC). The levels of pH and H2O2 were also analyzed. To assess the larvicidal effect of the CFS, this was tested pure and diluted (1:2 to 1:128) on T. canis larvae. High concentrations of lactic and acetic acids were detected in the CFS. The acidity of the pure CFS was observed at pH 3.8, remaining acidic at dilutions of 1:2 to 1:16. Regarding the in vitro larvicidal effect, 100% death of T. canis larvae was observed using the pure CFS and 1:2 dilution. Based on these results, it can be inferred that the presence of higher concentrations of organic acids and low pH of the medium contributed to the larvicidal activity of the CFS of L. acidophilus ATCC 4356. In addition, the maintenance of the larvicidal effect, even after dilution, suggests a greater chance of the larvicidal effect of this CFS against T. canis in vivo.
期刊介绍：
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.
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