大鼠死后肝脏和肌肉组织中乙醇生成的初步研究

IF 1.2 4区医学 Q3 MEDICINE, LEGAL

Journal of forensic and legal medicine Pub Date : 2024-02-01 DOI:10.1016/j.jflm.2024.102653

Qing Gao , Fanggang He , Hao Wang , Weisheng Huang , Hongmei Dong

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引用次数: 0

摘要

目的研究大鼠死后肝脏和肌肉组织中乙醇产生的特点及其与酒精同系物的关系。方法用顶空气相色谱仪火焰离子化检测法分析Sprague-Dawley大鼠死后0-20天的肝脏和肌肉组织，观察死后乙醇和5种选定的酒精同系物的产生情况。2.乙醛、1-丙醇和 3-甲基丁醛与死后乙醇同时产生，1-丁醇仅在第 11-20 天检测到；3.乙醛、1-丙醇和 3-甲基丁醛的浓度与乙醇的产生有关。根据乙醛、1-丙醇和 3-甲基丁醛构建了 15 个腐臭乙醇生成数学模型。肝脏中乙醛、1-丙醇和 3-甲基丁醛的生成趋势以及肌肉中 1-丙醇的生成趋势与乙醇的生成趋势一致，有可能被用作死后乙醇生成的生物标志物。要验证这一点，还需要更多的人体样本和数据分析。

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A primary study of ethanol production in postmortem liver and muscle tissue of rats

Objective

To study the characteristics of postmortem ethanol production and its relation with alcohol congeners in postmortem rat liver and muscle tissues.

Method

Postmortem liver and muscle tissues in Sprague–Dawley rats, from postmortem time interval (PMI) day 0–20, were analyzed via headspace gas chromatograph flame ionization detection to observe production of postmortem ethanol and 5 selected alcohol congeners.

Result

1. Putrid ethanol production increased gradually to a peak and then decreased with the prolongation of PMI; 2. Acetaldehyde, 1-propanol, and 3-methyl-butyraldehyde were produced along with postmortem ethanol; 1-butanol was only detected from day 11–20; 3. The concentrations of acetaldehyde, 1-propanol and 3-methyl-butyraldehyde was related with ethanol production. Fifteen mathematical models were constructed for putrid ethanol production based on acetaldehyde, 1-propanol, and 3-methyl-butyraldehyde.

Conclusion

A peak in postmortem ethanol production was identified. The production trends of acetaldehyde, 1-propanol, and 3-methyl-butyraldehyde in the liver, and of 1-propanol in muscle, were consistent with those of ethanol, and could potentially to be used as biomarkers of postmortem ethanol production. Further human samples and data analysis are needed to verify this.

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来源期刊

Journal of forensic and legal medicine MEDICINE, LEGAL-

CiteScore

2.70

自引率

6.70%

发文量

106

审稿时长

57 days

期刊介绍： The Journal of Forensic and Legal Medicine publishes topical articles on aspects of forensic and legal medicine. Specifically the Journal supports research that explores the medical principles of care and forensic assessment of individuals, whether adult or child, in contact with the judicial system. It is a fully peer-review hybrid journal with a broad international perspective. The Journal accepts submissions of original research, review articles, and pertinent case studies, editorials, and commentaries in relevant areas of Forensic and Legal Medicine, Context of Practice, and Education and Training. The Journal adheres to strict publication ethical guidelines, and actively supports a culture of inclusive and representative publication.